Genomic Identification and Functional Analysis of JHAMTs in the Pond Wolf Spider, Pardosa pseudoannulata .

Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, [2000-

Arthropod Proteins/*metabolism ; Methyltransferases/*metabolism ; Spiders/*metabolism; Animals ; Arthropod Proteins/genetics ; Female ; Gene Expression ; Juvenile Hormones/genetics ; Juvenile Hormones/metabolism ; Male ; Methyltransferases/genetics ; Phylogeny ; Spiders/genetics

Juvenile hormone (JH) plays a critical role in many physiological activities of Arthropoda. Juvenile hormone acid methyltransferase (JHAMT) is involved in the last steps of JH biosynthesis as an important rate-limiting enzyme. In recent studies, an increasing number of JHAMTs were identified in arthropods, but no JHAMT was reported in spiders. Herein, eight JHAMTs were identified in the pond wolf spider, Pardosa pseudoannulata , all containing the well conserved S-adenosyl-L-methionine binding motif. JHAMT-1 and the other seven JHAMTs were located at chromosome 13 and chromosome 1, respectively. Multiple alignment and phylogenetic analysis showed that JHAMT-1 was grouped together with insect JHAMTs independently and shared high similarities with insect JHAMTs compared to the other seven JHAMTs. In addition, JHAMT-1 , JHAMT-2 , and JHAMT-3 were highly expressed in the abdomen of spiderlings and could respond to the stimulation of exogenous farnesoic acid. Meanwhile, knockdown of these three JHAMTs caused the overweight and accelerated molting of spiderlings. These results demonstrated the cooperation of multi-JHAMTs in spider development and provided a new evolutionary perspective of the expansion of JHAMT in Arachnida.

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31772185 National Natural Science Foundation of China; 31972296 National Natural Science Foundation of China

Keywords: JHAMT; Pardosa pseudoannulata; RNA interference; gene expansion; phylogeny

0 (Arthropod Proteins)
0 (Juvenile Hormones)
EC 2.1.1.- (Methyltransferases)

Date Created: 20211113 Date Completed: 20211214 Latest Revision: 20211214

20240829

PMC8584100

10.3390/ijms222111721

34769151