Characteristics of Biotin lipoyl attachment and 2-oxoacid dehydrogenase acyltransferase of the parasitic nematode Haemonchus contortus and its modulatory functions on goat PBMCs in vitro.

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  • Additional Information
    • Source:
      Publisher: Wiley Country of Publication: England NLM ID: 7910948 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-3024 (Electronic) Linking ISSN: 01419838 NLM ISO Abbreviation: Parasite Immunol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Oxford, Wiley
    • Subject Terms:
      Haemonchiasis* ; Haemonchus*/genetics; Acyltransferases/metabolism ; Animals ; Biotin/metabolism ; Dihydrolipoamide Dehydrogenase/metabolism ; Goats/parasitology ; Helminth Proteins ; Keto Acids/metabolism
    • Abstract:
      Biotin lipoyl attachment and 2-oxoacid dehydrogenase acyltransferase (BLAODA), as an essential excretion of Haemonchus contortus (HcESPs), was identified to have antigenic functions. T helper-9 (Th9) cells secrete interleukin (IL)-9, a signature cytokine associated with tumour immunology, allergy and autoimmunity. Nonetheless, the understanding of modulatory functions of BLAODA on Th9 and other immune cells is limited. In this study, the BLAODA gene was cloned, and the recombinant (r) protein of BLAODA (rHcBLAODA) was expressed and immunoblotting was performed. The results revealed that HcBLAODA gene was successfully cloned and rHcBLAODA protein was expressed. The localization of rHcBLAODA was confirmed on the surface of gut sections from adult H. contortus. The rHcBLAODA protein capability to react precisely with anti-H. contortus antibodies were confirmed by immunoblotting and immunofluorescence assay (IFA). Further functional analysis showed that interaction of rHcBLAODA with host cells significantly enhanced Th9 cells generation, IL-9 expression, nitric oxide production and cell apoptosis while suppressing the cells proliferation and cells migration depending on the concentration. Overall, these findings suggest that rHcBLAODA protein could modulate the host immune response by inducing Th9 cells to secrete IL-9 cytokine in vitro.
      (© 2021 John Wiley & Sons Ltd.)
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    • Contributed Indexing:
      Keywords: Haemonchus contortus; Biotin lipoyl attachment and 2-oxoacid dehydrogenase acyltransferase; T helper-9 cells; modulatory functions; peripheral blood mononuclear cells
    • Molecular Sequence:
      GENBANK CDJ89966.1
    • Accession Number:
      0 (Helminth Proteins)
      0 (Keto Acids)
      6SO6U10H04 (Biotin)
      EC 1.8.1.4 (Dihydrolipoamide Dehydrogenase)
      EC 2.3.- (Acyltransferases)
    • Publication Date:
      Date Created: 20211021 Date Completed: 20220325 Latest Revision: 20220325
    • Publication Date:
      20221213
    • Accession Number:
      10.1111/pim.12895
    • Accession Number:
      34674283