Improved bioluminescence-based endotoxin measurement method using a salt-resistant luciferase mutant.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: United States NLM ID: 0370535 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0309 (Electronic) Linking ISSN: 00032697 NLM ISO Abbreviation: Anal Biochem Subsets: MEDLINE
    • Publication Information:
      Publication: <2000- > : San Diego, CA : Elsevier
      Original Publication: Orlando Fl : Academic Press
    • Subject Terms:
      Luminescent Measurements*; Endotoxins/*analysis ; Luciferases/*genetics ; Sodium Chloride/*chemistry; Luciferases/metabolism ; Mutation ; Salts/chemistry
    • Abstract:
      Endotoxin is a component of the cell wall of gram-negative bacteria and causes fever and shock symptoms upon entering the bloodstream. We previously demonstrated that the bioluminescence-based Limulus amebocyte lysate test is highly sensitive and rapid for measuring endotoxin. However, as the firefly luciferase reaction is inhibited in the presence of sodium chloride, the endotoxin detection method did not meet the validation guidelines under medical dialysis conditions (range of 75-125% of the measured values tested in water). Here, we used a salt-resistant luciferase mutant, which met the criteria for validation of endotoxin measurement.
      (Copyright © 2021 Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Detection; Dialysis; Endotoxin; Limulus amebocyte lysate test; Luciferase
    • Accession Number:
      0 (Endotoxins)
      0 (Salts)
      451W47IQ8X (Sodium Chloride)
      EC 1.13.12.- (Luciferases)
    • Publication Date:
      Date Created: 20211011 Date Completed: 20211228 Latest Revision: 20211228
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.ab.2021.114408
    • Accession Number:
      34634260