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High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging.
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- Additional Information
- Source:
Publisher: Elsevier Biomedical Country of Publication: Netherlands NLM ID: 8306883 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8359 (Electronic) Linking ISSN: 01677012 NLM ISO Abbreviation: J Microbiol Methods Subsets: MEDLINE
- Publication Information:
Original Publication: Amsterdam, The Netherlands : Elsevier Biomedical, c1983-
- Subject Terms:
- Abstract:
The crystal violet assay is widely used for biofilm quantitation despite its toxicity and variability. Here, we instead combine fluorescence labelling with the Cytation 5 multi-mode plate reader, to enable simultaneous acquisition of both quantitative and imaging biofilm data. This high-throughput method produces more robust data and provides information about morphology and spatial species organization within the biofilm.
(Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.)
- Contributed Indexing:
Keywords: Biofilm; Crystal Violet; Cytation5; Fluorescence labelling; High throughput assay; Imaging; Mixed species; Reproducibility
- Accession Number:
J4Z741D6O5 (Gentian Violet)
- Subject Terms:
Microbacterium oxydans; Paenibacillus amylolyticus; Stenotrophomonas rhizophila; Xanthomonas retroflexus
- Publication Date:
Date Created: 20211007 Date Completed: 20220119 Latest Revision: 20220119
- Publication Date:
20240829
- Accession Number:
10.1016/j.mimet.2021.106343
- Accession Number:
34619138
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