Growth and differentiation of cerebellar suspensions transplanted into the adult cerebellum of mice with heredodegenerative ataxia.

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  • Author(s): Sotelo C; Alvarado-Mallart RM
  • Source:
    Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1986 Feb; Vol. 83 (4), pp. 1135-9.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print Cited Medium: Print ISSN: 0027-8424 (Print) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, DC : National Academy of Sciences
    • Subject Terms:
      Cerebellar Ataxia/*therapy ; Purkinje Cells/*transplantation; Animals ; Axons/ultrastructure ; Cell Differentiation ; Cell Movement ; Cerebellar Ataxia/genetics ; Cerebellum/embryology ; Cerebellum/pathology ; Cerebellum/transplantation ; Dendrites/ultrastructure ; Graft Survival ; Mice ; Mice, Inbred C57BL ; Mice, Mutant Strains/genetics ; Mice, Mutant Strains/physiology ; Purkinje Cells/physiology ; Purkinje Cells/ultrastructure ; Synapses/ultrastructure
    • Abstract:
      Cell suspensions from cerebellar primordia of 12-day mouse embryos were grafted into the cerebellum of 4-month-old Purkinje cell degeneration (pcd) mutant mice and examined 2-3 months later. In contrast to those of nontreated mutants, all of the grafted cerebella exhibited Purkinje cells that had migrated into the molecular layer, where they were clustered over its superficial two-thirds. These Purkinje cells develop flattened dendritic trees perpendicular to bundles of parallel fibers. Ultrastructural examination of their synaptic inputs and outputs disclosed that (i) as in normal cerebella, climbing fibers and axons from basket and stellate cells synapse on thick dendrites, whereas parallel fibers almost exclusively contact the distal spiny branchlets, and (ii) a substantial number of Purkinje cell axons reach their appropriate targets in the deep cerebellar nuclei, where they establish synaptic connections on large and small neurons. These results indicate that embryonic Purkinje cells grafted into the cerebellum of adult mice with heredodegenerative ataxia integrate themselves very specifically into the cerebellar circuitry of the recipient mouse, where they can replace the missing Purkinje cells. They also provide a morphological basis favoring the notion of functional restorative capabilities of neural grafts in systems in which neurons are connected in an almost point-to-point manner.
    • References:
      J Histochem Cytochem. 1970 May;18(5):315-33. (PMID: 4192899)
      Proc Natl Acad Sci U S A. 1976 Jan;73(1):208-12. (PMID: 1061118)
      Nature. 1976 Aug 26;262(5571):787-90. (PMID: 958453)
      Nature. 1977 Nov 17;270(5634):245-7. (PMID: 593342)
      Philos Trans R Soc Lond B Biol Sci. 1977 Nov 2;281(978):1-28. (PMID: 22882)
      Prog Brain Res. 1978;48:149-70. (PMID: 746152)
      Dev Biol. 1985 Sep;111(1):129-37. (PMID: 2993082)
      Proc Natl Acad Sci U S A. 1979 Oct;76(10):5387-91. (PMID: 291955)
      Nature. 1981 Feb 5;289(5797):497-9. (PMID: 6258079)
      Nature. 1982 Nov 18;300(5889):260-2. (PMID: 7144881)
      Acta Physiol Scand Suppl. 1983;522:59-66. (PMID: 6586056)
      Proc Natl Acad Sci U S A. 1985 Feb;82(3):945-7. (PMID: 2579383)
      Brain Res. 1979 Oct 12;175(1):11-36. (PMID: 487138)
    • Publication Date:
      Date Created: 19860201 Date Completed: 19860328 Latest Revision: 20190501
    • Publication Date:
      20221208
    • Accession Number:
      PMC323026
    • Accession Number:
      10.1073/pnas.83.4.1135
    • Accession Number:
      3456566