Rapid detection and surveillance of cfiA-positive Bacteroides fragilis using matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Academic Press Country of Publication: England NLM ID: 9505216 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-8274 (Electronic) Linking ISSN: 10759964 NLM ISO Abbreviation: Anaerobe Subsets: MEDLINE
    • Publication Information:
      Publication: London : Academic Press
      Original Publication: London ; San Diego : Academic Press, c1995-
    • Subject Terms:
      Bacterial Typing Techniques* ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*/methods; Bacterial Proteins/*genetics ; Bacteroides Infections/*diagnosis ; Bacteroides Infections/*microbiology ; Bacteroides fragilis/*classification ; Bacteroides fragilis/*genetics ; beta-Lactamases/*genetics; Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/metabolism ; Bacteroides fragilis/drug effects ; Bacteroides fragilis/isolation & purification ; Disease Management ; Humans ; Microbial Sensitivity Tests ; beta-Lactamases/metabolism
    • Abstract:
      Objectives: To perform surveillance of cfiA-positive Bacteroides fragilis using new subtyping software module, MALDI Biotyper Subtyping Module (MBT Subtyping Module), on MALDI-TOF MS system, and to evaluate the detection ability of the module.
      Methods: cfiA-positive strains were presumed using the module against B. fragilis isolated between 2006 and 2019. The cfiA gene was confirmed using PCR. In cfiA-positive B. fragilis, the insertion sequence (IS) elements were examined and the MBT STAR-BL assay was performed to examine meropenem hydrolysis activity.
      Results: Of the 396 B. fragilis strains included, the MBT Subtyping Module detected 33 presumptive cfiA-positive strains (8.3%), of which 32 harbored the cfiA gene. The sensitivity and specificity of the MBT Subtyping Module for detecting cfiA-positive B. fragilis were 100.0% and 99.7%, respectively. Of the 32 strains harboring the cfiA gene, seven strains possessed IS elements, which were thought to induce high cfiA expression. Meropenem hydrolysis was detected in all seven strains that were positive for both cfiA and IS elements, and they exhibited resistance to meropenem and imipenem. The overall non-susceptibility rates to meropenem and imipenem were 84.8% and 36.4%, respectively, in the 33 presumptive cfiA-positive strains.
      Conclusion: The MBT Subtyping Module can detect cfiA-positive B. fragilis rapidly and accurately, supporting its use for surveillance of cfiA-positive B. fragilis in clinical settings.
      Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: K.K. and K.Y. received honoraria from Bruker Japan K.K. Y.K., K.O., N.U., K.S., H.H., I.K. and H.M. declare no conflicts of interest.
      (Copyright © 2021 Elsevier Ltd. All rights reserved.)
    • Contributed Indexing:
      Keywords: Carbapenem resistance; MALDI-TOF MS; Meropenem hydrolysis; Metallo-β-lactamase
    • Accession Number:
      0 (Anti-Bacterial Agents)
      0 (Bacterial Proteins)
      EC 3.5.2.6 (beta-Lactamases)
      EC 3.5.2.6 (carbapenemase)
    • Publication Date:
      Date Created: 20210919 Date Completed: 20220310 Latest Revision: 20220310
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.anaerobe.2021.102448
    • Accession Number:
      34537378