Airborne SARS-CoV-2 surveillance in hospital environment using high-flowrate air samplers and its comparison to surface sampling.

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  • Additional Information
    • Source:
      Publisher: Blackwell Country of Publication: England NLM ID: 9423515 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-0668 (Electronic) Linking ISSN: 09056947 NLM ISO Abbreviation: Indoor Air Subsets: MEDLINE
    • Publication Information:
      Publication: Oxford : Blackwell
      Original Publication: Copenhagen : Danish Technical Press, c1991-
    • Subject Terms:
      Air Microbiology* ; Air Pollution, Indoor* ; Hospitals*; SARS-CoV-2/*isolation & purification; COVID-19 ; Humans ; RNA, Viral ; Specimen Handling
    • Abstract:
      Reliable methods to detect the presence of SARS-CoV-2 at venues where people gather are essential for epidemiological surveillance to guide public policy. Communal screening of air in a highly crowded space has the potential to provide early warning on the presence and potential transmission of SARS-CoV-2 as suggested by studies early in the epidemic. As hospitals and public facilities apply varying degrees of restrictions and regulations, it is important to provide multiple methodological options to enable environmental SARS-CoV-2 surveillance under different conditions. This study assessed the feasibility of using high-flowrate air samplers combined with RNA extraction kit designed for environmental sample to perform airborne SARS-CoV-2 surveillance in hospital setting, tested by RT-qPCR. The success rate of the air samples in detecting SARS-CoV-2 was then compared with surface swab samples collected in the same proximity. Additionally, positive RT-qPCR samples underwent viral culture to assess the viability of the sampled SARS-CoV-2. The study was performed in inpatient ward environments of a quaternary care university teaching hospital in Singapore housing active COVID-19 patients within the period of February to May 2020. Two types of wards were tested, naturally ventilated open-cohort ward and mechanically ventilated isolation ward. Distances between the site of air sampling and the patient cluster in the investigated wards were also recorded. No successful detection of airborne SARS-CoV-2 was recorded when 50 L/min air samplers were used. Upon increasing the sampling flowrate to 150 L/min, our results showed a high success rate in detecting the presence of SARS-CoV-2 from the air samples (72%) compared to the surface swab samples (9.6%). The positive detection rate of the air samples along with the corresponding viral load could be associated with the distance between sampling site and patient. The furthest distance from patient with PCR-positive air samples was 5.5 m. The airborne SARS-CoV-2 detection was comparable between the two types of wards with 60%-87.5% success rate. High prevalence of the virus was found in toilet areas, both on surfaces and in air. Finally, no successful culture attempt was recorded from the environmental air or surface samples.
      (© 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
    • References:
      Epidemiol Infect. 2020 Jul 14;148:e154. (PMID: 32660668)
      Emerg Infect Dis. 2020 Jul;26(7):1583-1591. (PMID: 32275497)
      J Aerosol Sci. 2021 Feb;152:105693. (PMID: 33078030)
      PLoS One. 2018 Jul 18;13(7):e0200820. (PMID: 30020972)
      NPJ Biofilms Microbiomes. 2021 Apr 16;7(1):37. (PMID: 33863892)
      Aerosol Air Qual Res. 2020 Jun;20(6):1167-1171. (PMID: 33424954)
      Nature. 2021 Feb;590(7844):7. (PMID: 33531707)
      JAMA Netw Open. 2020 Dec 1;3(12):e2033232. (PMID: 33355679)
      Int J Infect Dis. 2020 Nov;100:476-482. (PMID: 32949774)
      Euro Surveill. 2020 Jan;25(3):. (PMID: 31992387)
      J Mol Diagn. 2020 Oct;22(10):1294-1299. (PMID: 32738298)
      Appl Environ Microbiol. 2018 Nov 15;84(23):. (PMID: 30217848)
      J Hosp Infect. 2021 Apr;110:89-96. (PMID: 33453351)
      Nature. 2021 Mar;591(7851):520-522. (PMID: 33737753)
      Sci Rep. 2020 Jul 29;10(1):12732. (PMID: 32728118)
      Emerg Infect Dis. 2020 Nov;26(11):2617-2624. (PMID: 32946369)
      Indoor Air. 2021 Sep;31(5):1639-1644. (PMID: 33876847)
      Nat Commun. 2020 Jul 8;11(1):3496. (PMID: 32641684)
      Nature. 2020 Jun;582(7813):557-560. (PMID: 32340022)
      Euro Surveill. 2020 Oct;25(42):. (PMID: 33094715)
      J Travel Med. 2020 Aug 20;27(5):. (PMID: 32662867)
      Indoor Air. 2022 Jan;32(1):e12930. (PMID: 34519380)
      Singapore Med J. 2022 Feb;63(2):61-67. (PMID: 32729311)
      Lancet Respir Med. 2020 Dec;8(12):e96. (PMID: 33137284)
      N Engl J Med. 2020 Dec 3;383(23):e129. (PMID: 33207089)
      Sci Total Environ. 2021 Jan 20;753:141710. (PMID: 32891988)
      Nat Commun. 2020 May 29;11(1):2800. (PMID: 32472043)
      Environ Int. 2020 Sep;142:105832. (PMID: 32521345)
      J Appl Microbiol. 2019 Dec;127(6):1596-1611. (PMID: 30974505)
    • Grant Information:
      National Medical Research Council grant MOH-000411; Freepoint Commodities, Pvt Ltd
    • Contributed Indexing:
      Keywords: COVID-19; communal testing; environmental surveillance; high-flowrate air sampling
    • Accession Number:
      0 (RNA, Viral)
    • Publication Date:
      Date Created: 20210914 Date Completed: 20220207 Latest Revision: 20221005
    • Publication Date:
      20231215
    • Accession Number:
      PMC8653264
    • Accession Number:
      10.1111/ina.12930
    • Accession Number:
      34519380