A sensor-based system for rapid on-site testing of microbial contamination in meat samples and carcasses.

Publisher: Oxford University Press Country of Publication: England NLM ID: 9706280 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2672 (Electronic) Linking ISSN: 13645072 NLM ISO Abbreviation: J Appl Microbiol Subsets: MEDLINE

Publication: 2022- : Oxford : Oxford University Press
Original Publication: Oxford : Published for the Society for Applied Bacteriology by Blackwell Science, c1997-

Food Microbiology* ; Meat Products*; Animals ; Cattle ; Colony Count, Microbial ; Food Contamination/analysis ; Meat

Aims: To develop an oxygen sensor-based method for testing total aerobic viable counts (TVC) in raw meat samples and cattle carcass swabs, which is rapid, simple, affordable, provides good sensitivity and analytical performance and allows on-site use.
Methods and Results: The test uses the same sample preparation procedure as the established plate counting TVC method for meat samples and carcasses, ISO4833-1:2013. After this liquid samples are transferred into standard 25-ml vials with built-in phosphorescent O 2  sensors and incubated on a block heater with hourly readings of sensor signals with a handheld reader, to determine signal threshold time (TT, hours) for each sample. The method is demonstrated with the quantification of TVC in industrial cuts of raw beef meat (CFU per g) and carcass swabs (CFU per cm ² ). Calibration curves were generated, which give the following analytical equations for calculating the TVC load in unknown samples from measured TT values: TVC [Log(CFU per cm ² )] = 7.83-0.73*TT(h) and TVC [Log(CFU per g)] = 8.74-0.70*TT(h) for the carcass swabs and meat samples respectively. The new tests show good correlation with the ISO methods, with correlation coefficients 0.85 and 0.83 respectively. The testing requires no dilutions, covers the ranges 2-7 Log(CFU per g) for the meat samples and 1-7 Log(CFU per cm ² ) for carcass swabs, and has time to result 1-10 h with faster detection of more contaminated samples.
Conclusions: The sensor-based testing demonstrates simplicity, high speed, sample throughput and automation. It can provide a straightforward replacement for the conventional TVC tests, which are time consuming, laborious and have time to result of 48-72 h.
Significance and Impact of the Study: The method(s) can be adopted by the meat industry and research labs, and used to improve microbial quality and safety of meat products and processes.
(© 2021 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.)

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DAFM/17/F/222 Department of Agriculture, Food and the Marine

Keywords: carcass swabs; optical oxygen sensing; rapid microbial testing; raw meat spoilage; respirometric assays; total viable counts

Date Created: 20210831 Date Completed: 20220121 Latest Revision: 20220121

20240829

10.1111/jam.15274

34464989