Chemical Transformation of Humic Acid Molecules under the Influence of Mineral, Fungal and Bacterial Fertilization in the Context of the Agricultural Use of Degraded Soils.

Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE

Original Publication: Basel, Switzerland : MDPI, c1995-

Agriculture*; Bacteria/*drug effects ; Fertilizers/*analysis ; Fungi/*drug effects ; Humic Substances/*analysis ; Minerals/*pharmacology ; Soil/*chemistry; Fluorescence ; Spectrophotometry, Ultraviolet

The main goal of this work was to study the structural transformation of humic acids (HAs) under the influence of selected strains of fungi ( Aspergillus niger and Paecilomyces lilacinus ) and bacteria ( Bacillus sp., Paenibacillus polymyxa and Bacillus amyloliquefaciens ) with/without the presence of NPK fertilizers. Two-year experiments were conducted on two different soils and HAs isolated from these soils were examined for structure, humification degree, and quantity using fluorescence and UV-Vis spectroscopy, elemental analysis, and extraction methods. Results showed that the applied additives contributed to the beneficial transformation of HAs, but effects differed for various soils. HAs from silty soil with higher organic carbon content showed simplification of their structure, and decreases in humification, molecular weight, and aromaticity under the influence of fungi and bacteria without NPK, and with NPK alone. With both fungi and NPK, increases in O/H and O/C atomic ratios indicated an increase in the number of O-containing functional groups. HAs from sandy soil did not show as many significant changes as did those from silty soil. Sandy soil exhibited a strong decline in HA content in the second year that was reduced/neutralized by the presence of fungi, bacteria, and NPK. Periodically observed fluorescence at ~300 nm/450 nm reflected formation of low-molecular HAs originating from the activity of microorganisms.

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BIOSTRATEG3/347464/5/NCBR/2017 "BIO-FERTIL" Narodowe Centrum Badań i Rozwoju

Keywords: bacterial effect on humification; fungal effect on humification; humification mechanism; structure of humic acids

0 (Fertilizers)
0 (Humic Substances)
0 (Minerals)
0 (Soil)

Date Created: 20210827 Date Completed: 20210916 Latest Revision: 20210916

20221213

PMC8399743

10.3390/molecules26164921

34443509