Molecular characterization of thioredoxin-interacting protein (TXNIP) from Megalobrama amblycephala and its potential roles in high glucose-induced inflammatory response.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
    • Publication Information:
      Publication: Amsterdam : Elsevier
      Original Publication: Guildford, Eng., IPC Science and Technology Press.
    • Subject Terms:
      Carrier Proteins/*genetics ; Cypriniformes/*genetics ; Liver/*metabolism ; Thioredoxins/*genetics; Animals ; Carrier Proteins/chemistry ; DNA, Complementary/genetics ; Glucose/adverse effects ; Hepatocytes/metabolism ; Humans ; Inflammation/genetics ; Inflammation/metabolism ; Insulin/genetics ; Interleukin-6/genetics ; Liver/drug effects ; Thioredoxins/metabolism
    • Abstract:
      This study aimed to characterize the full-length cDNA of thioredoxin-interacting protein (TXNIP) from Megalobrama amblycephala, and investigate its roles in high glucose (HC)-induced inflammatory response. The cDNA obtained covered 2706-bp with an open reading frame of 1203-bp encoding 400 amino acids, compared to Cyprinus carpio, it showed 89.96% homology. The highest expression of txnip was observed in head kidney followed by spleen and liver. After a 12-week feeding trial, high-carbohydrate diet remarkably increased txnip expression in liver and white muscle. Glucose administration resulted in a remarkably increased liver txnip expression, which peaked at 1 h. Thereafter, the expression decreased remarkably to the basal value at 12 h. However, insulin injection resulted in a significant decrease in txnip expression with minimum values attained at 2 h. Subsequently, it gradually increased to the normal values. Moreover, in the in-vitro study, over-expression of txnip along with remarkably increased il-1β and il-6 expression in hepatocytes, and its knockdown led to remarkably reduced il-1β expression. Furthermore, metformin treatment remarkably increased the cell viability and trx expression of hepatocytes under high glucose, while the opposite was true for ROS levels, LDH activity, the ALT/AST ratio, Txnip protein content and the transcriptions of txnip, tnfα and il-1β.
      (Copyright © 2021 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Glucose metabolism; Inflammation; Megalobrama amblycephala; Thioredoxin-interacting protein
    • Accession Number:
      0 (Carrier Proteins)
      0 (DNA, Complementary)
      0 (Insulin)
      0 (Interleukin-6)
      0 (TXNIP protein, human)
      52500-60-4 (Thioredoxins)
      IY9XDZ35W2 (Glucose)
    • Publication Date:
      Date Created: 20210815 Date Completed: 20211215 Latest Revision: 20211215
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.ijbiomac.2021.08.064
    • Accession Number:
      34391784