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Metabolomics provide new insights into mechanisms of Wolbachia-induced paternal defects in Drosophila melanogaster.
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- Author(s): Zhang HB;Zhang HB; Cao Z; Cao Z; Cao Z; Qiao JX; Qiao JX; Zhong ZQ; Zhong ZQ; Pan CC; Pan CC; Liu C; Liu C; Zhang LM; Zhang LM; Wang YF; Wang YF
- Source:
PLoS pathogens [PLoS Pathog] 2021 Aug 12; Vol. 17 (8), pp. e1009859. Date of Electronic Publication: 2021 Aug 12 (Print Publication: 2021).- Publication Type:
Journal Article; Research Support, Non-U.S. Gov't- Language:
English - Source:
- Additional Information
- Source: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101238921 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7374 (Electronic) Linking ISSN: 15537366 NLM ISO Abbreviation: PLoS Pathog Subsets: MEDLINE
- Publication Information: Original Publication: San Francisco, CA : Public Library of Science, c2005-
- Subject Terms: Metabolome* ; Phenotype* ; Reproduction*; Bacterial Infections/*complications ; Drosophila melanogaster/*metabolism ; Infertility, Male/*pathology ; Wolbachia/*physiology; Animals ; Bacterial Infections/metabolism ; Bacterial Infections/microbiology ; Drosophila melanogaster/growth & development ; Drosophila melanogaster/microbiology ; Female ; Infertility, Male/etiology ; Infertility, Male/metabolism ; Male
- Abstract: Wolbachia is a group of intracellular symbiotic bacteria that widely infect arthropods and nematodes. Wolbachia infection can regulate host reproduction with the most common phenotype in insects being cytoplasmic incompatibility (CI), which results in embryonic lethality when uninfected eggs fertilized with sperms from infected males. This suggests that CI-induced defects are mainly in paternal side. However, whether Wolbachia-induced metabolic changes play a role in the mechanism of paternal-linked defects in embryonic development is not known. In the current study, we first use untargeted metabolomics method with LC-MS to explore how Wolbachia infection influences the metabolite profiling of the insect hosts. The untargeted metabolomics revealed 414 potential differential metabolites between Wolbachia-infected and uninfected 1-day-old (1d) male flies. Most of the differential metabolites were significantly up-regulated due to Wolbachia infection. Thirty-four metabolic pathways such as carbohydrate, lipid and amino acid, and vitamin and cofactor metabolism were affected by Wolbachia infection. Then, we applied targeted metabolomics analysis with GC-MS and showed that Wolbachia infection resulted in an increased energy expenditure of the host by regulating glycometabolism and fatty acid catabolism, which was compensated by increased food uptake. Furthermore, overexpressing two acyl-CoA catabolism related genes, Dbi (coding for diazepam-binding inhibitor) or Mcad (coding for medium-chain acyl-CoA dehydrogenase), ubiquitously or specially in testes caused significantly decreased paternal-effect egg hatch rate. Oxidative stress and abnormal mitochondria induced by Wolbachia infection disrupted the formation of sperm nebenkern. These findings provide new insights into mechanisms of Wolbachia-induced paternal defects from metabolic phenotypes.
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Elife. 2019 Nov 27;8:. (PMID: 31774393) - Publication Date: Date Created: 20210812 Date Completed: 20211129 Latest Revision: 20231102
- Publication Date: 20231102
- Accession Number: PMC8384202
- Accession Number: 10.1371/journal.ppat.1009859
- Accession Number: 34383852
- Source:
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