Collagen and elastic fibres in acute and chronic liver injury.

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  • Additional Information
    • Source:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : Nature Publishing Group, copyright 2011-
    • Subject Terms:
      Collagen/*metabolism ; Elastic Tissue/*pathology ; Liver Cirrhosis/*pathology ; Liver Failure, Acute/*pathology; Adult ; Elastin/metabolism ; Extracellular Matrix/pathology ; Female ; Hepatocytes/pathology ; Humans ; Immunohistochemistry ; Liver Cirrhosis/metabolism ; Liver Failure, Acute/surgery ; Male ; Retrospective Studies ; Young Adult
    • Abstract:
      The histological distinction between acute and chronic liver injury is a challenging aspect of liver histopathology. It is traditionally based on the interpretation of morphological changes to the extracellular matrix (ECM) at sites of hepatocyte loss using histochemical stains. Our aim was to investigate whether immunohistochemistry and multiplexing for collagen type (I & III) and elastic fibres and a modified Victoria blue method could be helpful. We studied 43 livers removed at transplantation for acute liver failure (ALF, 20 cases) or cirrhosis (23) plus 8 normal controls. In ALF the periportal ECM was normal in 2 cases, contained mainly collagen I associated with a ductular reaction in 6 cases, and delicate elastic strands in 11 cases. Periportal deposition of mainly collagen I and mature elastic fibres was observed in cirrhosis. In ALF the perisinusoidal ECM was intact in 4 cases, collapsed or condensed but of normal composition (predominantly collagen III) in 2 cases, and collapsed and condensed containing mostly collagen I in 17 cases (7 including delicate immature elastic strands). In contrast, bridging fibrous septa of cirrhosis contained abundant collagen 1 and bundles of mature elastin. We propose the use of a scale and the use of immunohistochemistry and multiplexing in additional to histochemical stains to characterise the ECM changes in acute and chronic liver injury.
      (© 2021. The Author(s).)
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    • Grant Information:
      United Kingdom DH_ Department of Health
    • Accession Number:
      9007-34-5 (Collagen)
      9007-58-3 (Elastin)
    • Publication Date:
      Date Created: 20210716 Date Completed: 20211112 Latest Revision: 20211112
    • Publication Date:
      20231215
    • Accession Number:
      PMC8282791
    • Accession Number:
      10.1038/s41598-021-93566-1
    • Accession Number:
      34267266