A mitochondrial cytidine deaminase is responsible for C to U editing of tRNA Trp to decode the UGA codon in Trypanosoma brucei .

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  • Additional Information
    • Source:
      Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101235328 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1555-8584 (Electronic) Linking ISSN: 15476286 NLM ISO Abbreviation: RNA Biol Subsets: MEDLINE
    • Publication Information:
      Publication: 2015- : Philadelphia, PA : Taylor & Francis
      Original Publication: Georgetown, TX : Landes Bioscience,
    • Subject Terms:
      Codon, Terminator*; Cytidine/*genetics ; Cytidine Deaminase/*metabolism ; Mitochondria/*enzymology ; RNA, Protozoan/*genetics ; Trypanosoma brucei brucei/*genetics ; Uridine/*genetics; Amino Acid Sequence ; Base Sequence ; Cytidine/chemistry ; Cytidine Deaminase/genetics ; Mitochondria/genetics ; Nucleic Acid Conformation ; RNA, Mitochondrial/analysis ; RNA, Mitochondrial/genetics ; RNA, Protozoan/analysis ; RNA, Transfer, Trp ; Sequence Homology ; Trypanosoma brucei brucei/growth & development ; Trypanosoma brucei brucei/metabolism ; Uridine/chemistry
    • Abstract:
      In kinetoplastid protists, all mitochondrial tRNAs are encoded in the nucleus and imported from the cytoplasm to maintain organellar translation. This also applies to the tryptophanyl tRNA (tRNA Trp ) encoded by a single-copy nuclear gene, with a CCA anticodon to read UGG codon used in the cytosolic translation. Yet, in the mitochondrion it is unable to decode the UGA codon specifying tryptophan. Following mitochondrial import of tRNA Trp , this problem is solved at the RNA level by a single C34 to U34 editing event that creates the UCA anticodon, recognizing UGA. To identify the enzyme responsible for this critical editing activity, we scrutinized the genome of Trypanosoma brucei for putative cytidine deaminases as the most likely candidates. Using RNAi silencing and poisoned primer extension, we have identified a novel deaminase enzyme, named here TbmCDAT for mitochondrial Cytidine Deaminase Acting on tRNA, which is responsible for this organelle-specific activity in T. brucei . The ablation of TbmCDAT led to the downregulation of mitochondrial protein synthesis, supporting its role in decoding the UGA tryptophan codon.
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    • Contributed Indexing:
      Keywords: Mitochondrion; cytidine deaminase; editing; trypanosoma; tryptophanyl tRNA
    • Accession Number:
      0 (Codon, Terminator)
      0 (RNA, Mitochondrial)
      0 (RNA, Protozoan)
      0 (RNA, Transfer, Trp)
      5CSZ8459RP (Cytidine)
      EC 3.5.4.5 (Cytidine Deaminase)
      WHI7HQ7H85 (Uridine)
    • Publication Date:
      Date Created: 20210705 Date Completed: 20220225 Latest Revision: 20220225
    • Publication Date:
      20231215
    • Accession Number:
      PMC8677022
    • Accession Number:
      10.1080/15476286.2021.1940445
    • Accession Number:
      34224320