Intraocular pressure and injection forces during intravitreal injection into enucleated porcine eyes.

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  • Author(s): Allmendinger A;Allmendinger A; Butt YL; Butt YL; Mueller C; Mueller C
  • Source:
    European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V [Eur J Pharm Biopharm] 2021 Sep; Vol. 166, pp. 87-93. Date of Electronic Publication: 2021 Jun 06.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 9109778 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3441 (Electronic) Linking ISSN: 09396411 NLM ISO Abbreviation: Eur J Pharm Biopharm Subsets: MEDLINE
    • Publication Information:
      Publication: Amsterdam : Elsevier Science
      Original Publication: Stuttgart : Wissenschaftliche Verlagsgesellschaft, c1991-
    • Subject Terms:
    • Abstract:
      Injection of biological molecules into the intravitreous humor is of increasing interest for the treatment of posterior segment eye diseases such as age-related degenerative macular degeneration. The injection volume is limited by an increase in intraocular pressure (IOP) and 50-100 µL are typically used for most intravitreally (IVT) applied commercial products. Direct measurement of IOP is difficult and has not been studied dependent on solution properties and injection rates. We used an instrumental set-up to study IOP ex vivo using healthy enucleated porcine eyes. IOP was determined as a function of injection volume for viscosities between 1 and 100 mPas, injection rates of 0.1, 1, and 1.5 mL/min, and needle length and diameter (27/30G and 0.5/0.75″) using Dextran solutions. IOP increased exponentially for injection volumes larger than 100 µL. We did not observe differences in IOP dependent on viscosity, injection rate, and needle diameter. However, variability increased significantly for injection volumes larger than 100 µL and, unexpectedly, declined with higher viscosities. We demonstrate that the exponential increase in IOP is not reflected by injection force measurements for typical configurations that are used for IVT application. The present findings may guide injection volumes for intravitreal injection and inform injection force considerations during technical drug product development.
      (Copyright © 2021 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: Injection volume; Intraocular pressure; Intravitreal injection; Ocular delivery; Viscosity
    • Accession Number:
      0 (Dextrans)
      0 (Pharmaceutical Solutions)
      0 (Plasma Substitutes)
    • Publication Date:
      Date Created: 20210608 Date Completed: 20211231 Latest Revision: 20211231
    • Publication Date:
      20231215
    • Accession Number:
      10.1016/j.ejpb.2021.06.001
    • Accession Number:
      34102300