Immunophenotyping of the Medullary B Cell Compartment In Mouse Models.

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  • Additional Information
    • Source:
      Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
    • Publication Information:
      Publication: Totowa, NJ : Humana Press
      Original Publication: Clifton, N.J. : Humana Press,
    • Subject Terms:
      Cell Lineage* ; Flow Cytometry* ; Immunophenotyping*; B-Lymphocytes/*immunology; Animals ; B-Lymphocytes/metabolism ; Biomarkers/metabolism ; Mice ; Phenotype
    • Abstract:
      B cell development is a stepwise process encompassing several B cell precursor stages that can be phenotypically distinguished, and that is taking place in the bone marrow in adults. Interestingly, within the bone marrow B cell precursors coexist with the most differentiated actors of this lineage, the plasma cells. In this chapter, we describe a method to recover cells from the bone marrow and a flow cytometric approach to identify each subpopulation of the B cell lineage that resides within the bone marrow compartment. This protocol focuses on membrane markers to discriminate all the B cell subpopulations in order to preserve cell integrity during experimentation and for further analyses.
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    • Contributed Indexing:
      Keywords: B cell lineage; Bone marrow; Flow cytometry; Immature B cell; Mature B cell; Plasma cell; Pre-B cell; Prepro-B cell; Pro-B cell
    • Accession Number:
      0 (Biomarkers)
    • Publication Date:
      Date Created: 20210531 Date Completed: 20210810 Latest Revision: 20210810
    • Publication Date:
      20231215
    • Accession Number:
      10.1007/978-1-0716-1425-9_8
    • Accession Number:
      34057717