Human serum bilirubin fractionation in various hepatobiliary diseases by the newly developed high performance liquid chromatography.

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  • Additional Information
    • Source:
      Publisher: Japanese Society of Gastroenterology Country of Publication: Japan NLM ID: 0152744 Publication Model: Print Cited Medium: Print ISSN: 0435-1339 (Print) Linking ISSN: 04351339 NLM ISO Abbreviation: Gastroenterol Jpn Subsets: MEDLINE
    • Publication Information:
      Original Publication: Tokyo, Japanese Society of Gastroenterology.
    • Subject Terms:
      Biliary Tract Diseases/*blood ; Bilirubin/*blood ; Liver Diseases/*blood; Chromatography, High Pressure Liquid/methods ; Humans
    • Abstract:
      Serum Bilirubin was fractionated by newly developed reversed phase high performance liquid chromatography (HPLC) into 5 fractions: delta (delta-Bilirubin, B delta), gamma (bilirubin diglucuronide, BDG), beta (Bilirubin monoglucuronide, BMG), beta' ((Z, E,)- and/or (E, Z)-bilirubin IX alpha) and alpha ((Z, Z)-bilirubin IX alpha). Sera of healthy subjects and of patients with unconjugated hyperbilirubinemia showed predominantly alpha fraction with a small amount of beta' fraction. Trace amounts of delta fraction were detected in a few cases. The results of fractionation of serum bilirubin in 159 patients with various hepatobiliary diseases suggested that the ratios B delta/(B delta + BDG + BMG) and BMG/B delta can be useful parameters to follow patients with jaundice, compared with the reported B delta/total bilirubin which did not always reflect the jaundice stage, especially in cases with low serum bilirubin levels.
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    • Accession Number:
      RFM9X3LJ49 (Bilirubin)
    • Publication Date:
      Date Created: 19880601 Date Completed: 19880922 Latest Revision: 20190912
    • Publication Date:
      20231215
    • Accession Number:
      10.1007/BF02779469
    • Accession Number:
      3402701