Bioinformatic and Functional Characterization of Hsp70s in Myxococcus xanthus.

Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101674533 Publication Model: Electronic Cited Medium: Internet ISSN: 2379-5042 (Electronic) Linking ISSN: 23795042 NLM ISO Abbreviation: mSphere Subsets: MEDLINE

Original Publication: Washington, DC : American Society for Microbiology, [2015]-

Bacterial Proteins/*genetics ; Bacterial Proteins/*metabolism ; Computational Biology/*methods ; HSP72 Heat-Shock Proteins/*genetics ; HSP72 Heat-Shock Proteins/*metabolism ; Myxococcus xanthus/*chemistry ; Myxococcus xanthus/*genetics; HSP72 Heat-Shock Proteins/classification ; Myxococcus xanthus/metabolism ; Phylogeny ; Stress, Physiological ; Transcription, Genetic

Hsp70 proteins are among the most ubiquitous chaperones and play important roles in maintaining proteostasis and resisting environmental stress. Multiple copies of Hsp70s are widely present in eukaryotic cells with redundant and divergent functions, but they have been less well investigated in prokaryotes. Myxococcus xanthus DK1622 is annotated as having many hsp70 genes. In this study, we performed a bioinformatic analysis of Hsp70 proteins and investigated the functions of six hsp70 genes in DK1622, including two genes that encode proteins with the conserved PRK00290 domain (MXAN_3192 and MXAN_6671) and four genes that encode proteins with the cl35085 or cd10170 domain. We found that only MXAN_3192 is essential for cell survival and heat shock induction. MXAN_3192, compared with the other hsp70 genes, has a high transcriptional level, far exceeding that of any other hsp70 gene, which, however, is not the reason for its essentiality. Deletion of MXAN_6671 ( sglK ) led to multiple deficiencies in development, social motility, and oxidative resistance, while deletion of each of the other four hsp70 genes decreased sporulation and oxidative resistance. MXAN_3192 or sglK , but not the other genes, restored the growth deficiency of the E. coli dnaK mutant. Our results demonstrated that the PRK00290 proteins play a central role in the complex cellular functions of M. xanthus , while the other diverse Hsp70 superfamily homologues probably evolved as helpers with some unknown specific functions. IMPORTANCE Hsp70 proteins are highly conserved chaperones that occur in all kingdoms of life. Multiple copies of Hsp70s are often present in genome-sequenced prokaryotes, especially taxa with complex life cycles, such as myxobacteria. We investigated the functions of six hsp70 genes in Myxococcus xanthus DK1622 and demonstrated that the two Hsp70 proteins with the PRK00290 domain play a central role in complex cellular functions in M. xanthus , while other Hsp70 proteins probably evolved as helpers with some unknown specific functions.
(Copyright © 2021 Pan et al.)

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Keywords: DnaK proteins; Hsp70 superfamily; Myxococcus xanthus; social behavior; stress response; transcription

0 (Bacterial Proteins)
0 (HSP72 Heat-Shock Proteins)

Date Created: 20210520 Date Completed: 20211203 Latest Revision: 20211214

20221213

PMC8265645

10.1128/mSphere.00305-21

34011688