Guidelines for the measurement of vascular function and structure in isolated arteries and veins.

Publisher: American Physiological Society Country of Publication: United States NLM ID: 100901228 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1539 (Electronic) Linking ISSN: 03636135 NLM ISO Abbreviation: Am J Physiol Heart Circ Physiol Subsets: MEDLINE

Original Publication: Bethesda, Md. : American Physiological Society,

Arteries/*physiology ; Vasoconstriction/*physiology ; Vasodilation/*physiology ; Veins/*physiology; Animals ; Endothelium, Vascular/physiology ; Microscopy/methods ; Myography/methods ; Reproducibility of Results

The measurement of vascular function in isolated vessels has revealed important insights into the structural, functional, and biomechanical features of the normal and diseased cardiovascular system and has provided a molecular understanding of the cells that constitutes arteries and veins and their interaction. Further, this approach has allowed the discovery of vital pharmacological treatments for cardiovascular diseases. However, the expansion of the vascular physiology field has also brought new concerns over scientific rigor and reproducibility. Therefore, it is appropriate to set guidelines for the best practices of evaluating vascular function in isolated vessels. These guidelines are a comprehensive document detailing the best practices and pitfalls for the assessment of function in large and small arteries and veins. Herein, we bring together experts in the field of vascular physiology with the purpose of developing guidelines for evaluating ex vivo vascular function. By using this document, vascular physiologists will have consistency among methodological approaches, producing more reliable and reproducible results.

Comment in: Am J Physiol Heart Circ Physiol. 2022 Apr 1;322(4):H683-H684. (PMID: 35324332)
Comment in: Am J Physiol Heart Circ Physiol. 2022 Apr 1;322(4):H681-H682. (PMID: 35324333)
Comment in: Am J Physiol Heart Circ Physiol. 2022 Apr 1;322(4):H687-H688. (PMID: 35324334)
Comment in: Am J Physiol Heart Circ Physiol. 2022 Apr 1;322(4):H685-H686. (PMID: 35324335)

Arterioscler Thromb Vasc Biol. 2006 Jun;26(6):1297-302. (PMID: 16601235)
Sci Rep. 2014 Jun 16;4:5309. (PMID: 24930889)
Compr Physiol. 2017 Mar 16;7(2):321-382. (PMID: 28333376)
Curr Pharm Des. 2020;26(30):3723-3732. (PMID: 32303165)
Physiol Res. 2010;59(6):1033-1036. (PMID: 21208020)
Nature. 1998 Nov 19;396(6708):269-72. (PMID: 9834033)
Clin Sci (Lond). 2020 Jan 17;134(1):3-13. (PMID: 31898749)
Microvasc Res. 2015 Jan;97:156-8. (PMID: 25445029)
Pharmacol Res. 2019 Mar;141:276-290. (PMID: 30639374)
J Neurosci. 2016 Dec 14;36(50):12624-12639. (PMID: 27821575)
Front Physiol. 2018 Oct 15;9:1463. (PMID: 30374313)
Cardiovasc Diabetol. 2018 Jul 30;17(1):108. (PMID: 30060748)
Acta Physiol (Oxf). 2018 Dec;224(4):e13126. (PMID: 29896909)
Circ Res. 2000 Feb 18;86(3):341-6. (PMID: 10679487)
Cardiovasc Res. 2013 Jun 1;98(3):428-36. (PMID: 23417038)
Am J Physiol Heart Circ Physiol. 2020 Dec 1;319(6):H1387-H1397. (PMID: 33035443)
J Exerc Rehabil. 2020 Jun 30;16(3):207-215. (PMID: 32724777)
Arterioscler Thromb Vasc Biol. 2019 Feb;39(2):188-199. (PMID: 30567483)
J Physiol. 2017 Nov 1;595(21):6623-6634. (PMID: 28877347)
Indian J Pharmacol. 2010 Oct;42(5):329. (PMID: 21206631)
Front Physiol. 2018 Apr 06;9:341. (PMID: 29681862)
Br J Pharmacol. 2017 Oct;174(20):3561-3572. (PMID: 28150299)
Geroscience. 2021 Jun;43(3):1405-1422. (PMID: 33410092)
Am J Physiol Heart Circ Physiol. 2002 Dec;283(6):H2202-9. (PMID: 12388297)
Microcirculation. 2012 May;19(4):360-72. (PMID: 22335567)
Am J Physiol. 1995 Feb;268(2 Pt 2):H670-8. (PMID: 7864193)
Br J Pharmacol. 1994 Jun;112(2):579-87. (PMID: 7915613)
J Neurosci. 2015 Apr 1;35(13):5330-41. (PMID: 25834057)
Arterioscler Thromb Vasc Biol. 2020 Sep;40(9):2227-2243. (PMID: 32640901)
J Clin Invest. 2019 Jun 4;129(8):3140-3152. (PMID: 31162142)
Front Physiol. 2017 Feb 08;8:37. (PMID: 28228728)
Nat Commun. 2014 Jun 19;5:4099. (PMID: 24942009)
J Thromb Haemost. 2011 Jul;9 Suppl 1:118-29. (PMID: 21781247)
Front Physiol. 2019 Feb 21;10:99. (PMID: 30846942)
Microcirculation. 2001 Feb;8(1):45-55. (PMID: 11296852)
Am J Physiol. 1994 Jul;267(1 Pt 2):H326-32. (PMID: 8048598)
Pharmacol Res. 2021 Jan;163:105273. (PMID: 33197599)
Circ Res. 2011 Jun 10;108(12):1439-47. (PMID: 21512160)
Am J Physiol Heart Circ Physiol. 2005 Jul;289(1):H322-9. (PMID: 15722407)
Sci Signal. 2017 Jul 04;10(486):. (PMID: 28676489)
J Thorac Cardiovasc Surg. 2005 Oct;130(4):1130-6. (PMID: 16214530)
J Physiol. 2019 Aug;597(15):3833-3852. (PMID: 31165480)
Hypertension. 1986 Apr;8(4):344-8. (PMID: 2870025)
Clin Sci (Lond). 2020 Dec 11;134(23):3195-3211. (PMID: 33215657)
Hypertension. 2020 Aug;76(2):393-403. (PMID: 32594801)
Am J Physiol Heart Circ Physiol. 2005 Oct;289(4):H1326-34. (PMID: 15863456)
J Pharmacol Exp Ther. 1979 Oct;211(1):59-67. (PMID: 490327)
Am J Physiol. 1994 Mar;266(3 Pt 2):H952-8. (PMID: 8160843)
Hypertension. 2009 Feb;53(2):307-12. (PMID: 19114645)
Cardiovasc Res. 2001 Mar;49(4):828-37. (PMID: 11230983)
J Neurosci. 2015 Sep 30;35(39):13463-74. (PMID: 26424891)
Prostaglandins Med. 1980 Aug;5(2):123-30. (PMID: 6251495)
Am J Physiol Heart Circ Physiol. 2003 Jul;285(1):H104-11. (PMID: 12649071)
J Physiol. 1902 May 28;28(3):220-31. (PMID: 16992618)
Microcirculation. 2012 Feb;19(2):99-114. (PMID: 21895845)
Br J Pharmacol. 2006 Jan;147 Suppl 1:S252-7. (PMID: 16402111)
Arterioscler Thromb Vasc Biol. 2018 Mar;38(3):542-554. (PMID: 29301784)
Br J Anaesth. 2003 Apr;90(4):514-6. (PMID: 12644429)
Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):18174-9. (PMID: 23071308)
Am J Physiol Heart Circ Physiol. 2002 Dec;283(6):H2260-7. (PMID: 12388265)
Hypertension. 2001 Feb;37(2):313-21. (PMID: 11230291)
Pharmacol Res. 2016 Nov;113(Pt A):384-394. (PMID: 27639600)
J Cereb Blood Flow Metab. 2016 Mar;36(3):492-512. (PMID: 26661232)
Microcirculation. 2017 Aug;24(6):. (PMID: 28370794)
Am J Physiol Heart Circ Physiol. 2020 Jun 1;318(6):H1410-H1419. (PMID: 32357115)
Development. 2017 Jan 1;144(1):83-94. (PMID: 28049691)
J Neurosurg. 1991 Sep;75(3):433-9. (PMID: 1869945)
Am J Physiol. 1990 Jan;258(1 Pt 2):H127-33. (PMID: 2105665)
Clin Exp Hypertens A. 1991;13(2):277-96. (PMID: 2065467)
Clin Sci (Lond). 2012 Jan;122(1):1-12. (PMID: 21910690)
Am J Physiol. 1995 Nov;269(5 Pt 2):H1590-6. (PMID: 7503253)
Nature. 2008 Dec 11;456(7223):745-9. (PMID: 18971930)
Cardiovasc Res. 1998 May;38(2):316-31. (PMID: 9709392)
FASEB J. 2002 Jul;16(9):1057-63. (PMID: 12087067)
J Vis Exp. 2018 Feb 26;(132):. (PMID: 29553547)
Adv Physiol Educ. 2003 Dec;27(1-4):201-6. (PMID: 14627618)
Am J Physiol Heart Circ Physiol. 2014 Feb 15;306(4):H485-95. (PMID: 24337457)
J Physiol. 2016 Dec 1;594(23):7027-7047. (PMID: 27531064)
Am J Physiol Heart Circ Physiol. 2021 Feb 1;320(2):H584-H592. (PMID: 33185115)
Physiol Res. 2014;63(1):143-6. (PMID: 24665830)
Exp Physiol. 2005 Jan;90(1):131-40. (PMID: 15466455)
J Neurosci. 2015 May 27;35(21):8245-57. (PMID: 26019339)
Annu Rev Physiol. 2019 Feb 10;81:89-111. (PMID: 30742784)
Basic Res Cardiol. 2011 Nov;106(6):1123-34. (PMID: 21744279)
Am J Physiol. 1989 Feb;256(2 Pt 2):H383-90. (PMID: 2492768)
Am J Physiol Heart Circ Physiol. 2010 Feb;298(2):H466-76. (PMID: 19966050)
J Vasc Res. 2008;45(3):211-21. (PMID: 18087168)
Mt Sinai J Med. 2004 Sep;71(4):274-80. (PMID: 15365594)
PLoS One. 2016 May 17;11(5):e0155377. (PMID: 27187080)
Physiol Res. 2010;59(3):331-338. (PMID: 19681660)
J Vasc Res. 2007;44(4):264-72. (PMID: 17377397)
Biochim Biophys Acta. 2014 Nov;1842(11):2106-2119. (PMID: 25045854)
Physiol Rev. 1999 Apr;79(2):387-423. (PMID: 10221985)
Ann Biomed Eng. 2015 Nov;43(11):2760-70. (PMID: 25986954)
Physiology (Bethesda). 2009 Feb;24:58-71. (PMID: 19196652)
J Physiol. 2012 Apr 1;590(7):1757-70. (PMID: 22310311)
Carcinogenesis. 2018 Jan 12;39(1):21-25. (PMID: 28968787)
Arterioscler Thromb Vasc Biol. 2015 Jun;35(6):1393-400. (PMID: 25838427)
J Vis Exp. 2018 Apr 9;(134):. (PMID: 29683445)
Am J Physiol Heart Circ Physiol. 2007 Mar;292(3):H1390-7. (PMID: 17071727)
Am J Physiol. 1969 Jun;216(6):1343-50. (PMID: 5786720)
Am J Physiol. 1981 Jul;241(1):H108-116. (PMID: 7195654)
Am J Physiol. 1986 Jun;250(6 Pt 2):H1145-9. (PMID: 3487253)
Am J Physiol Regul Integr Comp Physiol. 2019 Jul 1;317(1):R149-R159. (PMID: 31091154)
Am J Physiol Heart Circ Physiol. 2017 Jul 1;313(1):H164-H178. (PMID: 28432057)
J Cereb Blood Flow Metab. 2015 Jul;35(7):1127-36. (PMID: 25757753)
Anat Sci Int. 2010 Mar;85(1):23-30. (PMID: 19488686)
Am J Physiol Heart Circ Physiol. 2018 Aug 1;315(2):H189-H205. (PMID: 29631368)
Circulation. 2013 Jul 2;128(1):9-18. (PMID: 23685742)
J Appl Physiol (1985). 2012 Oct;113(7):1128-40. (PMID: 22837170)
Circ Res. 2019 May 10;124(10):1473-1481. (PMID: 30900949)
Front Neuroenergetics. 2011 Dec 29;3:9. (PMID: 22232599)
J Physiol. 2020 Sep;598(18):3793-3801. (PMID: 32666574)
Circulation. 2018 Jul 3;138(1):67-79. (PMID: 29371216)
Am J Physiol Heart Circ Physiol. 2004 Mar;286(3):H1107-13. (PMID: 14644761)
J Pharmacol Pharmacother. 2013 Oct;4(4):303-6. (PMID: 24250214)
Br J Pharmacol. 1980 Jun;69(2):207-14. (PMID: 6254589)
Br J Pharmacol. 1980;71(2):585-96. (PMID: 7470763)
Cell Calcium. 2013 Feb;53(2):112-9. (PMID: 23177664)
Proc Natl Acad Sci U S A. 2011 Oct 25;108(43):17827-31. (PMID: 22006332)
J Pharmacol Exp Ther. 2018 Apr;365(1):60-71. (PMID: 29348267)
Sci Rep. 2020 Feb 4;10(1):1807. (PMID: 32019956)
Integr Blood Press Control. 2014 May 23;7:29-34. (PMID: 24910511)
J Am Heart Assoc. 2020 Nov 3;9(21):e016929. (PMID: 33073641)
Hypertension. 2016 Nov;68(5):1236-1244. (PMID: 27572153)
Cell Mol Life Sci. 2019 Feb;76(4):777-789. (PMID: 30448891)
Nature. 1976 Apr 15;260(5552):617-9. (PMID: 1264225)
PLoS One. 2007 Sep 05;2(9):e827. (PMID: 17786199)

R01HL139585 HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI); P20 GM130459 United States GM NIGMS NIH HHS; R01HL121871 HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI); DK115255 HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK); R61 NS115132 United States NS NINDS NIH HHS; K99HL151889 HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI); R01HL151413 HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI); R00HL116769 HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI); R01 HD037831 United States HD NICHD NIH HHS; R01HL091905 HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI); R01HL088554 HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI); R01 HL139585 United States HL NHLBI NIH HHS; P20GM130459 HHS | NIH | National Institute of General Medical Sciences (NIGMS); R01 HL135901 United States HL NHLBI NIH HHS; RF1 NS110044 United States NS NINDS NIH HHS; R01ES014639 HHS | NIH | National Institute of Environmental Health Sciences (NIEHS); U24 DK076169 United States DK NIDDK NIH HHS; S10OD023438 HHS | NIH | NIH Office of the Director (OD); R01HL137112 HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI); R01HL135901 HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI); R01HL146914 HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI); R00 HL116769 United States HL NHLBI NIH HHS; K99 HL151889 United States HL NHLBI NIH HHS; U24 DK115255 United States DK NIDDK NIH HHS; R21 EB026518 United States EB NIBIB NIH HHS; R01 HL149762 United States HL NHLBI NIH HHS; DK076169 HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK); R01NS082521 HHS | NIH | National Institute of Neurological Disorders and Stroke (NINDS); R01 HL146054 United States HL NHLBI NIH HHS; R21EB026518 HHS | NIH | National Institute of Biomedical Imaging and Bioengineering (NIBIB); R01 HL123301 United States HL NHLBI NIH HHS; P01 HL134604 United States HL NHLBI NIH HHS; R00GM118885 HHS | NIH | National Institute of General Medical Sciences (NIGMS); R01 HL091905 United States HL NHLBI NIH HHS; RF1NS110044 HHS | NIH | National Institute of Neurological Disorders and Stroke (NINDS); R01HL142808 HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI); R61NS115132 HHS | NIH | National Institute of Neurological Disorders and Stroke (NINDS); R01HL146562 HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI); R01 HL088105 United States HL NHLBI NIH HHS; R01 HL137852 United States HL NHLBI NIH HHS; R35 HL155008 United States HL NHLBI NIH HHS; R01 HL137112 United States HL NHLBI NIH HHS; R01HL149762 HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI); R01HL123301 HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI); R01 HL146914 United States HL NHLBI NIH HHS; R01 HL142808 United States HL NHLBI NIH HHS; R01 HL088554 United States HL NHLBI NIH HHS; R01HD037831 HHS | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD); R01HL146054 HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI); R01 HL146562 United States HL NHLBI NIH HHS; R44 HL121871 United States HL NHLBI NIH HHS; R01HL088105 HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI); R01 ES014639 United States ES NIEHS NIH HHS; P01HL134604 HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI); R01HL137852 HHS | NIH | National Heart, Lung, and Blood Institute (NHLBI); S10 OD023438 United States OD NIH HHS; R01 HL151413 United States HL NHLBI NIH HHS; R41 HL121871 United States HL NHLBI NIH HHS; R00 GM118885 United States GM NIGMS NIH HHS

Keywords: arteries; contraction; methods; relaxation; veins

Date Created: 20210514 Date Completed: 20210914 Latest Revision: 20220716

20240628

PMC8321813

10.1152/ajpheart.01021.2020

33989082