MicroRNA775 regulates intrinsic leaf size and reduces cell wall pectin levels by targeting a galactosyltransferase gene in Arabidopsis.

Publisher: Oxford University Press Country of Publication: England NLM ID: 9208688 Publication Model: Print Cited Medium: Internet ISSN: 1532-298X (Electronic) Linking ISSN: 10404651 NLM ISO Abbreviation: Plant Cell Subsets: MEDLINE

Publication: 2021- : [Oxford] : Oxford University Press
Original Publication: Rockville, MD : American Society of Plant Physiologists, c1989-

Arabidopsis/*metabolism ; Arabidopsis Proteins/*metabolism ; Cell Wall/*metabolism ; Galactosyltransferases/*metabolism ; Pectins/*metabolism ; Plant Leaves/*metabolism ; Plants, Genetically Modified/*metabolism; Arabidopsis Proteins/genetics ; Galactosyltransferases/genetics ; Gene Expression Regulation, Plant ; Plants, Genetically Modified/genetics

Plants possess unique primary cell walls made of complex polysaccharides that play critical roles in determining intrinsic cell and organ size. How genes responsible for synthesizing and modifying the polysaccharides in the cell wall are regulated by microRNAs (miRNAs) to control plant size remains largely unexplored. Here we identified 23 putative cell wall-related miRNAs, termed as CW-miRNAs, in Arabidopsis thaliana and characterized miR775 as an example. We showed that miR775 post-transcriptionally silences GALT9, which encodes an endomembrane-located galactosyltransferase belonging to the glycosyltransferase 31 family. Over-expression of miR775 and deletion of GALT9 led to significantly enlarged leaf-related organs, primarily due to increased cell size. Monosaccharide quantification, confocal Raman imaging, and immunolabeling combined with atomic force microscopy revealed that the MIR775A-GALT9 circuit modulates pectin levels and the elastic modulus of the cell wall. We also showed that MIR775A is directly repressed by the transcription factor ELONGATED HYPOCOTYL5 (HY5). Genetic analysis confirmed that HY5 is a negative regulator of leaf size that acts through the HY5-MIR775A-GALT9 repression cascade to control pectin levels. These findings demonstrate that miR775-regulated cell wall remodeling is an integral determinant of intrinsic leaf size in A. thaliana. Studying other CW-miRNAs would provide more insights into cell wall biology.
(© American Society of Plant Biologists 2021. All rights reserved. For permissions, please email: [email protected].)

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0 (Arabidopsis Proteins)
89NA02M4RX (Pectins)
EC 2.4.1.- (Galactosyltransferases)

Date Created: 20210506 Date Completed: 20210806 Latest Revision: 20220302

20221213

PMC8136896

10.1093/plcell/koaa049

33955485