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An acoustic field-based conformal transfection system for improving the gene delivery efficiency.
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- Additional Information
- Source:
Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101593571 Publication Model: Print Cited Medium: Internet ISSN: 2047-4849 (Electronic) Linking ISSN: 20474830 NLM ISO Abbreviation: Biomater Sci Subsets: MEDLINE
- Publication Information:
Original Publication: Cambridge, UK : Royal Society of Chemistry
- Subject Terms:
- Abstract:
Ultrasound-activated microbubble destruction is a promising platform for gene delivery due to the low toxicity, non-invasiveness, and high specificity. However, the gene transfection efficiency is still low, especially for suspension cells. It is desirable to develop a universal gene delivery tool that overcomes the drawbacks existing in ultrasound-mediated methods. Here, we present a three-dimensional acoustic field-based conformal transfection (AFCT) system by designing a Sono-hole that can fit the three-dimensional acoustic field to maximally utilize the acoustic energy from bubble cavitation, thus greatly promoting the gene delivery efficiency. Surprisingly, compared with the traditional two-dimensional transfection system, the gene transfection efficiency of the AFCT system increased by more than 3 times, achieving nearly 30%. The parameters including acoustic pressure, duration, duty cycle, DNA concentrations, and bubble kinds were optimized to obtain higher gene transfection. In conclusion, our study provides an effective ultrasound-based gene delivery approach for gene transfection, especially for suspension-cultured cells.
- Publication Date:
Date Created: 20210506 Date Completed: 20210607 Latest Revision: 20210607
- Publication Date:
20250114
- Accession Number:
10.1039/d1bm00251a
- Accession Number:
33954320
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