Proteome Analysis with Classical 2D-PAGE.

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  • Additional Information
    • Source:
      Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
    • Publication Information:
      Publication: Totowa, NJ : Humana Press
      Original Publication: Clifton, N.J. : Humana Press,
    • Subject Terms:
      Electrophoresis, Gel, Two-Dimensional* ; Electrophoresis, Polyacrylamide Gel* ; Isoelectric Focusing* ; Proteome* ; Proteomics*; Proteins/*analysis; Animals ; Fluorescent Dyes ; Humans ; Hydrogen-Ion Concentration ; Luminescent Measurements ; Research Design ; Staining and Labeling
    • Abstract:
      Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) is based on the combination of two orthogonal separation techniques. In the first dimension, proteins are separated by their isoelectric point, a technique known as isoelectric focusing (IEF). There are two important variants of IEF, which are carrier-ampholine (CA)-based IEF and immobilized pH-gradient (IPG)-based IEF. In the second dimension, proteins are further separated by their electrophoretic mobility using SDS-PAGE. Finally, proteins can be visualized and quantified by different staining procedures such as Coomassie, silver staining, or fluorescence labeling. This article gives detailed protocols for 2D-PAGE, using both CA- and IPG-based separation in the first dimension.
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    • Contributed Indexing:
      Keywords: Carrier ampholyte system (CA); Immobilized pH gradient (IPG); Isoelectric focusing (IEF); Sodium dodecyl sulfate (SDS); Two-dimensional polyacrylamide gel Electrophoresis (2D-PAGE)
    • Accession Number:
      0 (Fluorescent Dyes)
      0 (Proteins)
      0 (Proteome)
    • Publication Date:
      Date Created: 20210505 Date Completed: 20210623 Latest Revision: 20210623
    • Publication Date:
      20240829
    • Accession Number:
      10.1007/978-1-0716-1024-4_5
    • Accession Number:
      33950483