Differential inflammatory responses associated with Leishmania major and L tropica in culture.

Publisher: Wiley Country of Publication: England NLM ID: 7910948 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-3024 (Electronic) Linking ISSN: 01419838 NLM ISO Abbreviation: Parasite Immunol Subsets: MEDLINE

Original Publication: Oxford, Wiley

Leishmania major* ; Leishmania tropica* ; Leishmaniasis, Cutaneous*/epidemiology; Cytokines ; Humans ; Leukocytes, Mononuclear

Background: Anthroponotic cutaneous leishmaniasis (ACL) due to Leishmania tropica and zoonotic CL (ZCL) due to L major have different clinical and epidemiological features.
Objectives: To determine whether pro-inflammatory cytokines are involved in diverse pathogenicity of Leishmania species causing CL.
Patients/methods: The capacity of L major/L tropica to modulate expression of IL-1β, IL-8 (CXCL8), IFN-γ, TNF-α and MCP-1 (CCL2) in peripheral blood mononuclear cells (PBMCs) and monocyte-derived macrophages (MDMs) was evaluated by real-time RT-PCR technique.
Results: PBMCs from both ZCL and ACL cases expressed significantly higher IFN-γ (P < .001) and TNF-α (P < .05) compared with healthy controls (HC). PBMCs from ACL patients expressed significantly higher IL-1β and IL-8 compared with ZCL patients and HC when stimulated with live L major or L tropica promastigotes (P < .001). After 4 and 10 hours, L major-infected MDMs expressed significantly higher IFN-γ (P < .05), and after 10 hours, L tropica-infected MDMs expressed significantly higher IL-1β, IFN-γ and IL-8 compared with noninfected cells (P < .05).
Conclusions: This study shows differential parasite-mediated stimulations of the inflammatory response with L major vs L tropica ex vivo. Pro-inflammatory cytokines particularly IL-8 (CXCL8) and IL-1β might contribute in diverse clinical features of CL such as longer duration of lesion persistence in ACL patients.
(© 2021 John Wiley & Sons Ltd.)

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Keywords: Leishmania major; Leishmania tropica; cutaneous leishmaniasis; interleukin-1β; interleukin-8; pro-inflammatory cytokines

0 (Cytokines)

Date Created: 20210429 Date Completed: 20211125 Latest Revision: 20211125

20240829

10.1111/pim.12841

33914948