Time-course transcriptomic analysis of Petunia ×hybrida leaves under water deficit stress using RNA sequencing.

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  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
      Gene Expression Regulation, Plant* ; Transcriptome*; Dehydration/*genetics ; Petunia/*genetics ; RNA-Seq/*methods ; Stress, Physiological/*genetics; Cluster Analysis ; Down-Regulation ; Gene Ontology ; Homeostasis/genetics ; Petunia/metabolism ; Plant Growth Regulators/genetics ; Plant Growth Regulators/metabolism ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Plant Stomata/genetics ; Plant Stomata/metabolism ; Signal Transduction/genetics ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Up-Regulation
    • Abstract:
      Water deficit limits plant growth and development, resulting in quality loss of horticultural crops. However, there is limited information on gene regulation and signaling pathways related to water deficit stress response at multiple time points. The objective of this research was to investigate global gene expression patterns under water deficit stress to provide an insight into how petunia (Petunia ×hybrida 'Mitchell Diploid') responded in the process of stress. Nine-week-old petunias were irrigated daily or placed under water stress by withholding water. Stressed plants reduced stomatal conductance after five days of water deficit, indicating they perceived stress and initiated stress response mechanisms. To analyze transcriptomic changes at the early stage of water deficit, leaf tissue samples were collected 1, 3, and 5 days after water was withheld for RNA sequencing. Under water deficit stress, 154, 3611, and 980 genes were upregulated and 41, 2806, and 253 genes were downregulated on day 1, 3, and 5, respectively. Gene Ontology analysis revealed that redox homeostasis processes through sulfur and glutathione metabolism pathways, and hormone signal transduction, especially abscisic acid and ethylene, were enriched under water deficit stress. Thirty-four transcription factor families were identified, including members of AP2/ERF, NAC, MYB-related, C2H2, and bZIP families, and TFs in AP2/ERF family was the most abundant in petunia. Interestingly, only one member of GRFs was upregulated on day 1, while most of TFs were differentially expressed on day 3 and/or 5. The transcriptome data from this research will provide valuable molecular resources for understanding the early stages of water stress-responsive networks as well as engineering petunia with enhanced water stress tolerance.
      Competing Interests: The authors have declared that no competing interests exist.
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    • Accession Number:
      0 (Plant Growth Regulators)
      0 (Plant Proteins)
      0 (Transcription Factors)
    • Publication Date:
      Date Created: 20210426 Date Completed: 20211006 Latest Revision: 20231111
    • Publication Date:
      20231215
    • Accession Number:
      PMC8075263
    • Accession Number:
      10.1371/journal.pone.0250284
    • Accession Number:
      33901201