Simiao Wan attenuates monosodium urate crystal-induced arthritis in rats through contributing to macrophage M2 polarization.

Publisher: Elsevier Sequoia Country of Publication: Ireland NLM ID: 7903310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7573 (Electronic) Linking ISSN: 03788741 NLM ISO Abbreviation: J Ethnopharmacol Subsets: MEDLINE

Publication: Limerick : Elsevier Sequoia
Original Publication: Lausanne, Elsevier Sequoia.

Anti-Inflammatory Agents/*pharmacology ; Anti-Inflammatory Agents/*therapeutic use ; Antirheumatic Agents/*pharmacology ; Antirheumatic Agents/*therapeutic use ; Arthritis, Experimental/*prevention & control ; Drugs, Chinese Herbal/*pharmacology ; Drugs, Chinese Herbal/*therapeutic use ; Macrophages/*drug effects; Animals ; Anti-Inflammatory Agents/chemistry ; Antirheumatic Agents/chemistry ; Arginase/metabolism ; Arthritis, Experimental/pathology ; Drugs, Chinese Herbal/chemistry ; Edema/chemically induced ; Edema/drug therapy ; Edema/pathology ; Extremities/pathology ; Gait/drug effects ; I-kappa B Proteins/metabolism ; Inflammation/chemically induced ; Inflammation/drug therapy ; Inflammation/pathology ; Interleukin-10/metabolism ; Interleukin-1beta/metabolism ; Janus Kinase 2/metabolism ; Macrophage Activation/drug effects ; Macrophages/metabolism ; Male ; Neoplasm Proteins/metabolism ; Nitric Oxide Synthase Type II/metabolism ; Nucleocytoplasmic Transport Proteins/metabolism ; Rats, Sprague-Dawley ; STAT3 Transcription Factor/metabolism ; Synovial Membrane/drug effects ; Synovial Membrane/metabolism ; Uric Acid/toxicity ; Rats

Ethnopharmacological Relevance: Simiao Wan (SMW) is a classical traditional Chinese medicine (TCM) prescription to empirically treat gouty arthritis (GA) in TCM clinical practice. However, the potential mechanisms of SMW on GA are not fully evaluated.
Aim of Study: The aim of this study is to investigate the role of macrophage polarization in the anti-GA activity of SMW.
Materials and Methods: Rats were intragastricly treated with SMW for consecutive 7 days. On day 6, monosodium urate (MSU) crystal-induced arthritis (MIA) in the ankle joint was prepared. Paw volume, gait score and histological score were measured. Levels of interleukin (IL)-1β and IL-10 in serum were detected by enzyme-linked immunosorbent assay. Expressions of inducible nitric oxide synthase (iNOS), arginase (Arg)-1, phosphorylated (p)-p65, inhibitor of nuclear factor (NF)-κB (IκB)α, p-signal transducer and transcription activator (STAT)3 and p-Janus kinase (JAK)2 in synovial tissues were determined by Western blot.
Results: The elevated paw volume, gait score and histological score in MIA rats were significantly decreased by SMW treatment. Meanwhile, SMW significantly decreased the IL-1β level and increased the IL-10 level in serum of MIA rats. Furthermore, SMW reduced the expressions of iNOS, p-p65 and enhanced the expressions of Arg-1, IκBα, p-STAT3 and p-JAK2 in synovial tissues of MIA rats.
Conclusions: The results suggest that SMW attenuates the inflammation in MIA rats through promoting macrophage M2 polarization.
(Copyright © 2021 Elsevier B.V. All rights reserved.)

Keywords: Gouty arthritis; MSU-Induced arthritis; Macrophage polarization; Simiao Wan

0 (Anti-Inflammatory Agents)
0 (Antirheumatic Agents)
0 (Drugs, Chinese Herbal)
0 (I-kappa B Proteins)
0 (IL1B protein, rat)
0 (Interleukin-1beta)
0 (Neoplasm Proteins)
0 (Nucleocytoplasmic Transport Proteins)
0 (STAT3 Transcription Factor)
0 (Stat3 protein, rat)
0 (p65 oncofetal mRNA transport protein, rat)
0 (simiaowan)
130068-27-8 (Interleukin-10)
268B43MJ25 (Uric Acid)
EC 1.14.13.39 (Nitric Oxide Synthase Type II)
EC 1.14.13.39 (Nos2 protein, rat)
EC 2.7.10.2 (Jak2 protein, rat)
EC 2.7.10.2 (Janus Kinase 2)
EC 3.5.3.1 (Arginase)

Date Created: 20210424 Date Completed: 20211203 Latest Revision: 20240226

20240226

10.1016/j.jep.2021.114123

33894285