Component-resolved diagnosis using guinea-pig allergens elucidates allergen sensitization profiles in allergy to furry animals.

Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 8906443 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2222 (Electronic) Linking ISSN: 09547894 NLM ISO Abbreviation: Clin Exp Allergy Subsets: MEDLINE

Original Publication: Oxford : Blackwell Scientific Publications, c1989-

Allergens/*immunology ; Dander/*immunology ; Hypersensitivity/*diagnosis ; Immunoglobulin E/*immunology ; Lipocalins/*immunology; Adult ; Animals ; Cats ; Cross Reactions/immunology ; Dogs ; Female ; Guinea Pigs ; Humans ; Hypersensitivity/immunology ; Male ; Pets

Background: Furry animals are an important source of indoor allergens. Diagnosis of allergy to small pets such as guinea-pigs still relies on animal dander extracts which do not allow to define the primary sensitization source.
Objective: To identify major guinea-pig allergens and to evaluate their potential as marker allergens for in vitro IgE-diagnosis in comparison with dander extracts.
Methods: A group of patients allergic to guinea-pig (n = 29) and a group of patients allergic to cat and dog (n = 30) were recruited for the study. A panel of four guinea-pig lipocalin allergens was expressed as recombinant proteins in E. coli. Specific IgE were quantified by ImmunoCAP and ELISA.
Results: The combination of 4 guinea-pig lipocalin allergens, including 2 new lipocalins, Cav p 1.0201 and Cav p 6.0101, and the previously characterized lipocalins Cav p 2 and Cav p 3, enabled the identification of 90% of all patients allergic to guinea-pig. The vast majority had specific IgE to Cav p 1 (83%). Cav p 6 shares 54% sequence identity with Fel d 4 and Can f 6 and was found to be IgE-cross-reactive with these allergens. In the group of cat- and dog-allergic patients, 73% had also specific IgE to guinea-pig dander. However, only 27% of the cat /dog-allergic patients had specific IgE to any of the non-cross-reactive guinea-pig allergens Cav p 1, Cav p 2 or Cav p 3. The high prevalence of IgE to guinea-pig dander could be explained by IgE-cross-reactivity among serum albumins and certain lipocalins.
Conclusions and Clinical Relevance: The availability of specific allergen markers is essential for the assessment of primary sensitization, especially in polysensitized patients. The proposed panel of guinea-pig allergens Cav p 1, Cav p 2 and Cav p 3 is a first step to component-resolved IgE-diagnosis of allergy to small furry pets.
(© 2021 The Authors. Clinical & Experimental Allergy published by John Wiley & Sons Ltd.)

Allergy. 2002 May;57(5):417-22. (PMID: 11972481)
Allergy. 2009 Oct;64(10):1498-506. (PMID: 19772515)
J Allergy Clin Immunol. 2016 Mar;137(3):813-21.e7. (PMID: 26686472)
J Allergy Clin Immunol. 2009 Feb;123(2):513-5. (PMID: 18980777)
Curr Allergy Asthma Rep. 2017 Aug 22;17(9):64. (PMID: 28831729)
J Allergy Clin Immunol. 1995 Dec;96(6 Pt 1):951-9. (PMID: 8543754)
J Allergy Clin Immunol. 2012 Apr;129(4):1149-51. (PMID: 22104604)
Pediatr Allergy Immunol. 2016 May;27 Suppl 23:1-250. (PMID: 27288833)
Allergy. 2012 Jun;67(6):751-7. (PMID: 22515174)
Br J Ind Med. 1992 Jan;49(1):41-7. (PMID: 1733454)
Allergy. 2011 Nov;66(11):1500-1. (PMID: 21790648)
Clin Exp Allergy. 2013 Feb;43(2):233-41. (PMID: 23331564)
Int Arch Allergy Immunol. 2015;166(1):30-40. (PMID: 25765367)
J Allergy Clin Immunol. 2015 Mar;135(3):616-25. (PMID: 25282018)
Allergol Select. 2017 Aug 4;1(2):214-221. (PMID: 30402618)
Clin Exp Allergy. 2011 Jun;41(6):899-908. (PMID: 21518038)
Front Immunol. 2014 Mar 03;5:76. (PMID: 24624129)

Keywords: Guinea-pig; IgE-diagnosis; allergen; lipocalin

0 (Allergens)
0 (Can f 6 allergen, Canis familiaris)
0 (Cav p 1 allergen, guinea pig)
0 (Fel d 4 allergen, Felis domesticus)
0 (Lipocalins)
37341-29-0 (Immunoglobulin E)

Date Created: 20210402 Date Completed: 20220131 Latest Revision: 20220131

20250114

PMC8251889

10.1111/cea.13873

33797108