Anticancer Cardenolides from the aerial parts of Calortopis procera .

Publisher: De Gruyter Country of Publication: Germany NLM ID: 8912155 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1865-7125 (Electronic) Linking ISSN: 03410382 NLM ISO Abbreviation: Z Naturforsch C J Biosci Subsets: MEDLINE

Publication: Berlin : De Gruyter
Original Publication: Tübingen : Verlag der Zeitschrift für Naturforschung, 1986-

Antineoplastic Agents, Phytogenic/*chemistry ; Apocynaceae/*chemistry ; Cardenolides/*chemistry; Antineoplastic Agents, Phytogenic/isolation & purification ; Antineoplastic Agents, Phytogenic/pharmacology ; Apocynaceae/metabolism ; Cardenolides/isolation & purification ; Cardenolides/pharmacology ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Humans ; Magnetic Resonance Spectroscopy ; Molecular Conformation ; Plant Components, Aerial/chemistry ; Plant Components, Aerial/metabolism ; Plant Extracts/chemistry

Column chromatography (CC) analysis of methanol and butanol extracts of the aerial parts of Calortopis procera as well as the methanol extract of its latex, led to the isolation of 8 cardenolides, of which the structures were elucidated by NMR and HRESIMS spectroscopy. They also revealed several triterpenes and flavonoid glycoside. Based on the antiproliferative activity reported for cardenolides, the activity of calotropin and calotoxin was tested against two common cancer cell lines, human triple-negative breast cancer cell line (MDA-MB-231) and human lung adenocarcinoma cell line (A549). The high toxicity of the latex also encouraged performing the same test on the same cancer cell lines. The anti-proliferative activity of calotropin and calotoxin was compared to the methanol extract and the wax of the latex. The results showed that calotropin and calotoxin have significant cytotoxicity against MDA-MB-231 and A549 cell lines ranging from 0.046 to 0.072 μM compared to the methanol extract and the wax of its latex ranging from 0.47 to 58.41 μM. Moreover, the results showed lower toxicity of all treatments to the human skin fibroblasts compared to the toxicity to both MDA-MB-231 and A549 cancer cells lines except the higher toxicity of Methanolic extracts of C. procera latex to the MDA-MB-231 cells. In conclusion, C. procera is a medicinal plant with a wide spectrum of cardinolides including calotropin and calotoxin, which are promising agents for targeted cancer phytotherapy.
(© 2021 Walter de Gruyter GmbH, Berlin/Boston.)

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Keywords: C. procera; breast cancer; calotoxin; calotropin; latex; lung cancer

0 (Antineoplastic Agents, Phytogenic)
0 (Cardenolides)
0 (Plant Extracts)
3XK21U1BZS (calotropin)

Date Created: 20210326 Date Completed: 20211103 Latest Revision: 20211103

20221213

10.1515/znc-2020-0281

33770827