Nutrient availability regulates proline/alanine transporters in Trypanosoma brucei.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • Additional Information
    • Source:
      Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1083-351X (Electronic) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE
    • Publication Information:
      Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology
      Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology
    • Subject Terms:
    • Abstract:
      Trypanosoma brucei is a species of unicellular parasite that can cause severe diseases in livestock and humans, including African trypanosomiasis and Chagas disease. Adaptation to diverse environments and changes in nutritional conditions is essential for T. brucei to establish an infection when changing hosts or during invasion of different host tissues. One such adaptation is the ability of T. brucei to rapidly switch its energy metabolism from glucose metabolism in the mammalian blood to proline catabolism in the insect stages and vice versa. However, the mechanisms that support the parasite's response to nutrient availability remain unclear. Using RNAseq and qRT-PCR, we investigated the response of T. brucei to amino acid or glucose starvation and found increased mRNA levels of several amino acid transporters, including all genes of the amino acid transporter AAT7-B subgroup. Functional characterization revealed that AAT7-B members are plasma membrane-localized in T. brucei and when expressed in Saccharomyces cerevisiae supported the uptake of proline, alanine, and cysteine, while other amino acids were poorly recognized. All AAT7-B members showed a preference for proline, which is transported with high or low affinity. RNAi-mediated AAT7-B downregulation resulted in a reduction of intracellular proline concentrations and growth arrest under low proline availability in cultured procyclic form parasites. Taken together, these results suggest a role of AAT7-B transporters in the response of T. brucei to proline starvation and proline catabolism.
      Competing Interests: Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.
      (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
    • References:
      Mol Biochem Parasitol. 2009 Aug;166(2):172-82. (PMID: 19443056)
      BMC Genomics. 2014 Oct 20;15:911. (PMID: 25331479)
      Curr Biol. 2016 Jan 25;26(2):161-172. (PMID: 26725202)
      BMC Evol Biol. 2007 Feb 23;7:26. (PMID: 17319943)
      PLoS One. 2014 Mar 17;9(3):e92028. (PMID: 24637744)
      Sci Rep. 2015 Nov 09;5:16289. (PMID: 26549185)
      J Biol Chem. 2005 Mar 25;280(12):11902-10. (PMID: 15665328)
      F1000Res. 2017 May 16;6:. (PMID: 28620452)
      BMC Genomics. 2009 Oct 19;10:482. (PMID: 19840382)
      Exp Parasitol. 1999 Apr;91(4):341-8. (PMID: 10092478)
      Z Parasitenkd. 1981;66(1):17-24. (PMID: 7324539)
      J Proteome Res. 2014 Jun 6;13(6):2796-806. (PMID: 24792668)
      PLoS Pathog. 2010 Nov 24;6(11):e1001204. (PMID: 21124824)
      Pathogens. 2018 Apr 01;7(2):. (PMID: 29614775)
      FEBS J. 2009 Dec;276(23):7187-99. (PMID: 19895576)
      Genome Biol. 2009;10(3):R25. (PMID: 19261174)
      Antimicrob Agents Chemother. 2013 Jun;57(6):2768-79. (PMID: 23571546)
      Mol Cell Proteomics. 2011 Oct;10(10):M111.010538. (PMID: 21685506)
      J Insect Physiol. 1976;22(2):309-13. (PMID: 1249439)
      PLoS Pathog. 2017 Dec 21;13(12):e1006679. (PMID: 29267392)
      Plant J. 1993 Dec;4(6):993-1002. (PMID: 8281191)
      J Eukaryot Microbiol. 2002 Nov-Dec;49(6):441-6. (PMID: 12503677)
      Mol Biochem Parasitol. 2011 Jan;175(1):91-4. (PMID: 20851719)
      J Parasitol. 1989 Dec;75(6):985-9. (PMID: 2614608)
      Front Biosci (Landmark Ed). 2012 Jan 01;17(1):349-74. (PMID: 22201748)
      J Biol Chem. 2000 Dec 22;275(51):40174-9. (PMID: 11013266)
      Acta Trop. 1978 Sep;35(3):287-90. (PMID: 31783)
      J Parasitol. 1977 Feb;63(1):15-24. (PMID: 321737)
      Nucleic Acids Res. 2018 Jul 2;46(W1):W537-W544. (PMID: 29790989)
      PLoS One. 2012;7(5):e36619. (PMID: 22574199)
      J Biol Chem. 2006 Mar 17;281(11):7197-204. (PMID: 16407306)
      Nucleic Acids Res. 2010 Jan;38(Database issue):D457-62. (PMID: 19843604)
      Acta Trop. 1974;31(1):83-8. (PMID: 4151040)
      Mol Microbiol. 1997 May;24(3):607-16. (PMID: 9179853)
      J Chromatogr. 1984 Dec 7;336(1):93-104. (PMID: 6396315)
      J Biol Chem. 2020 Jun 12;295(24):8331-8347. (PMID: 32354742)
      J Mol Biol. 1980 Jun 5;139(4):691-704. (PMID: 6251229)
      Mol Cell Proteomics. 2015 Apr;14(4):1113-26. (PMID: 25670805)
      Plant Cell. 1996 Aug;8(8):1437-46. (PMID: 8776904)
      Genes Dev. 2004 Nov 15;18(22):2798-811. (PMID: 15545633)
      Nat Biotechnol. 2005 Sep;23(9):1089-91. (PMID: 16151400)
      Metabolomics. 2015;11(6):1721-1732. (PMID: 26491423)
      Mol Biochem Parasitol. 2003 Feb;126(2):219-30. (PMID: 12615321)
      Front Cell Infect Microbiol. 2013 Nov 14;3:78. (PMID: 24294594)
      PLoS Pathog. 2016 Apr 04;12(4):e1005494. (PMID: 27043018)
      PLoS Negl Trop Dis. 2013 Dec 19;7(12):e2587. (PMID: 24367711)
      Proc Natl Acad Sci U S A. 2020 Feb 4;117(5):2613-2621. (PMID: 31964820)
      Parasitology. 2015 Feb;142 Suppl 1:S40-56. (PMID: 25068268)
      BMC Genomics. 2009 Sep 11;10:427. (PMID: 19747379)
      PLoS Pathog. 2010 Jun 03;6(6):e1000926. (PMID: 20532213)
      mSphere. 2018 Jul 18;3(4):. (PMID: 30021880)
      Sci Rep. 2018 May 21;8(1):7960. (PMID: 29785042)
      Gene. 1985;38(1-3):205-14. (PMID: 3905514)
      Biochem J. 1996 Nov 1;319 ( Pt 3):691-7. (PMID: 8920968)
      Mol Biochem Parasitol. 1999 Nov 30;104(2):311-22. (PMID: 10593184)
      J Biol Chem. 2008 Jun 13;283(24):16342-54. (PMID: 18430732)
      Eur J Biochem. 1987 May 4;164(3):601-6. (PMID: 3552672)
      Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772)
      Mol Biochem Parasitol. 1999 Mar 15;99(1):89-101. (PMID: 10215027)
      Trends Parasitol. 2007 Apr;23(4):149-58. (PMID: 17320480)
      Nucleic Acids Res. 2010 Aug;38(15):4946-57. (PMID: 20385579)
      Biochem J. 2005 Oct 15;391(Pt 2):425-32. (PMID: 16008527)
      Microb Genom. 2015 Oct 30;1(4):e000033. (PMID: 28348817)
      Methods Enzymol. 2002;350:3-41. (PMID: 12073320)
      Nat Commun. 2015 Mar 26;6:6646. (PMID: 25808593)
      Nucleic Acids Res. 2009 Jan;37(Database issue):D603-10. (PMID: 18953024)
      PLoS One. 2017 Jan 3;12(1):e0168775. (PMID: 28045943)
      Bioessays. 2003 Jan;25(1):39-46. (PMID: 12508281)
      Exp Parasitol. 1961 Sep;11:161-75. (PMID: 14007246)
      Biol Rev Camb Philos Soc. 2017 Aug;92(3):1675-1687. (PMID: 27739621)
      Nat Biotechnol. 2019 Apr;37(4):420-423. (PMID: 30778233)
      Curr Opin Microbiol. 2008 Aug;11(4):345-51. (PMID: 18621142)
      PLoS Pathog. 2014 Jan;10(1):e1003886. (PMID: 24453978)
      mSphere. 2018 Oct 31;3(5):. (PMID: 30381351)
      PLoS Pathog. 2017 Jan 23;13(1):e1006158. (PMID: 28114403)
      Biochem J. 2013 Jan 15;449(2):555-66. (PMID: 22994895)
      Genome Res. 2003 Sep;13(9):2178-89. (PMID: 12952885)
      Biochem J. 2014 Oct 1;463(1):9-18. (PMID: 24988048)
      Mol Microbiol. 2011 Jan;79(1):94-108. (PMID: 21166896)
      J Biol Chem. 2002 Sep 6;277(36):32849-54. (PMID: 12095995)
      Elife. 2016 Sep 22;5:. (PMID: 27653219)
      PLoS One. 2016 Dec 21;11(12):e0168877. (PMID: 28002435)
      Parasitology. 2008 Apr;135(5):585-94. (PMID: 18371239)
      Mol Microbiol. 2006 Apr;60(1):30-8. (PMID: 16556218)
      Mol Biochem Parasitol. 2010 Jul;172(1):52-5. (PMID: 20302889)
      Trends Parasitol. 2020 Mar;36(3):266-278. (PMID: 32014419)
      BMC Genomics. 2009 Aug 07;10:370. (PMID: 19664227)
      Mol Biochem Parasitol. 1998 Jun 1;93(2):251-62. (PMID: 9662709)
      Biochem J. 1993 Apr 1;291 ( Pt 1):297-301. (PMID: 8471048)
      Plant J. 2002 Mar;29(6):717-31. (PMID: 12148530)
      Proteomics. 2008 Jan;8(1):83-99. (PMID: 18095354)
      Parasitology. 1972 Jun;64(3):379-87. (PMID: 5039338)
      PLoS Pathog. 2010 Aug 05;6(8):e1001037. (PMID: 20700444)
      Mol Cell Proteomics. 2014 Jul;13(7):1769-86. (PMID: 24741115)
      Ann Trop Med Parasitol. 1956;50(3):334-44. (PMID: 13363298)
      Trends Parasitol. 2017 Feb;33(2):80-82. (PMID: 27863903)
      PLoS Negl Trop Dis. 2017 Oct 26;11(10):e0006025. (PMID: 29073150)
      Mol Biochem Parasitol. 1983 Sep;9(1):47-57. (PMID: 6669160)
      Eukaryot Cell. 2009 Aug;8(8):1307-11. (PMID: 19542311)
      FASEB J. 2017 Oct;31(10):4649-4660. (PMID: 28679527)
      Mol Cell Biol. 2014 Dec;34(24):4447-63. (PMID: 25266656)
      Yeast. 1991 Oct;7(7):691-2. (PMID: 1776359)
      Acta Trop. 1979 Sep;36(3):289-92. (PMID: 43092)
      Nucleic Acids Res. 2007 Jul;35(Web Server issue):W585-7. (PMID: 17517783)
      BMC Genomics. 2018 Apr 2;19(1):227. (PMID: 29606092)
      Trends Parasitol. 2006 Apr;22(4):168-74. (PMID: 16504583)
      Comp Biochem Physiol A Comp Physiol. 1974 Oct 1;49(2A):215-22. (PMID: 4153811)
      PLoS Pathog. 2016 Feb 24;12(2):e1005439. (PMID: 26910529)
      Life Sci Alliance. 2019 Sep 30;2(5):. (PMID: 31570514)
      Genetics. 2007 Jun;176(2):877-90. (PMID: 17435223)
      Anal Bioanal Chem. 2010 Nov;398(5):2059-69. (PMID: 20824428)
      FEBS Lett. 1995 Aug 21;370(3):264-8. (PMID: 7656990)
      BMC Genomics. 2009 Oct 26;10:495. (PMID: 19857263)
      Open Biol. 2015 Jan;5(1):140197. (PMID: 25567099)
      Trends Parasitol. 2017 Apr;33(4):276-284. (PMID: 28007406)
      Int J Parasitol. 1973 Sep;3(5):691-704. (PMID: 4354962)
      Nucleic Acids Res. 2014 Apr;42(6):3623-37. (PMID: 24442674)
      Eur J Biochem. 1996 Nov 1;241(3):779-86. (PMID: 8944766)
      Nature. 2018 Nov;563(7729):121-125. (PMID: 30333624)
      R Soc Open Sci. 2017 May 3;4(5):170095. (PMID: 28573017)
      Anal Biochem. 1987 Apr;162(1):156-9. (PMID: 2440339)
    • Contributed Indexing:
      Keywords: Trypanosoma brucei; amino acid; amino acid transport; cell metabolism; energy metabolism; gene expression; parasite metabolism; transcriptomics; transporter
    • Accession Number:
      0 (Amino Acid Transport Systems, Neutral)
      9040-98-6 (proline transporter)
      OF5P57N2ZX (Alanine)
    • Publication Date:
      Date Created: 20210322 Date Completed: 20210818 Latest Revision: 20240402
    • Publication Date:
      20240402
    • Accession Number:
      PMC8094907
    • Accession Number:
      10.1016/j.jbc.2021.100566
    • Accession Number:
      33745971