Expression dynamics of vascular endothelial markers: endoglin and syndecan-1 in predicting dengue disease outcome.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: United States NLM ID: 101280339 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1810 (Electronic) Linking ISSN: 18781810 NLM ISO Abbreviation: Transl Res Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York, N.Y. : Elsevier, [2006]-
    • Subject Terms:
      Prognosis*; Biomarkers/*blood ; Dengue/*blood ; Endoglin/*blood ; Syndecan-1/*blood; Endoglin/genetics ; Endothelial Cells/physiology ; Female ; Gene Expression ; Humans ; India ; Male ; Prospective Studies ; RNA, Messenger/blood ; Severity of Illness Index ; Syndecan-1/genetics
    • Abstract:
      Plasma leakage is a hallmark process in dengue viral (DENV) infection that occurs due to the loss of vascular integrity in endothelial cells. Endoglin (ENG) and Syndecan-1 (SDC-1) are released by activated endothelial cells; however, the complete dynamics of its expression at the gene and protein levels during the course of DENV infection remains unknown. In the present study, we quantified the mRNA and soluble protein levels of ENG and SDC-1 in dengue cases during febrile, defervescence, and convalescence stages in Dengue without Warning Sign (DWOW-15), Dengue with Warning Sign (DWW-22), and Severe Dengue cases (SD-10) compared to nondengue Other Febrile Illness (OFI-10) and healthy control (HC-8). Respective protein and mRNA levels along with clinical characters were further analyzed for their efficacy in predicting disease outcomes using Support Vector Machine (SVM). We observed a steady and significant (P ≤ 0.01) increase in the levels of protein and mRNA of both the ENG and SDC-1 towards defervescence which is considered a critical phase in both severe and non-severe dengue cases. Importantly during the critical phase, the levels were significantly higher (P ≤ 0.001) in SD cases compared to DWW, DWOW, and OFI controls. However, at the time of admission (febrile), no such significant changes were observed within dengue, OFI, and healthy controls. SVM analysis revealed that the serum levels of ENG and SDC-1 along with other clinical symptoms could predict the disease severity with 100% accuracy. Based on the results we have proposed a mechanism on how ENG and SDC-1 could be involved in vascular dysfunction rather than just being a biomarker.
      (Copyright © 2021 Elsevier Inc. All rights reserved.)
    • Accession Number:
      0 (Biomarkers)
      0 (Endoglin)
      0 (RNA, Messenger)
      0 (SDC1 protein, human)
      0 (Syndecan-1)
    • Publication Date:
      Date Created: 20210210 Date Completed: 20210804 Latest Revision: 20210804
    • Publication Date:
      20231215
    • Accession Number:
      10.1016/j.trsl.2021.02.001
    • Accession Number:
      33567345