pH-dependent delivery of chlorhexidine from PGA grafted mesoporous silica nanoparticles at resin-dentin interface.

Publisher: BioMed Central Country of Publication: England NLM ID: 101152208 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-3155 (Electronic) Linking ISSN: 14773155 NLM ISO Abbreviation: J Nanobiotechnology Subsets: MEDLINE

Original Publication: London : BioMed Central, 2003-

Dentin*/diagnostic imaging; Anti-Bacterial Agents/*pharmacology ; Chlorhexidine/*pharmacology ; Composite Resins/*chemistry ; Glycolates/*pharmacology ; Nanoparticles/*chemistry ; Silicon Dioxide/*chemistry; Anti-Bacterial Agents/chemistry ; Biofilms/drug effects ; Chlorhexidine/chemistry ; Dental Materials/chemistry ; Drug Delivery Systems ; Drug Liberation ; Glycolates/chemistry ; Humans ; Hydrogen-Ion Concentration ; Materials Testing ; Tensile Strength

Background: A low pH environment is created due to the production of acids by oral biofilms that further leads to the dissolution of hydroxyapatite crystal in the tooth structure significantly altering the equilibrium. Although the overall bacterial counts may not be eradicated from the oral cavity, however, synthesis of engineered anti-bacterial materials are warranted to reduce the pathogenic impact of the oral biofilms. The purpose of this study was to synthesize and characterize chlorhexidine (CHX)-loaded mesoporous silica nanoparticles (MSN) grafted with poly-L-glycolic acid (PGA) and to test the in vitro drug release in various pH environments, cytotoxicity, and antimicrobial capacity. In addition, this study aimed to investigate the delivery of CHX-loaded/MSN-PGA nanoparticles through demineralized dentin tubules and how these nanoparticles interact with tooth dentin after mixing with commercial dentin adhesive for potential clinical application.
Results: Characterization using SEM/TEM and EDX confirmed the synthesis of CHX-loaded/MSN-PGA. An increase in the percentage of drug encapsulation efficiency from 81 to 85% in CHX loaded/MSN and 92-95% in CHX loaded/MSN-PGA proportionately increased with increasing the amount of CHX during the fabrication of nanoparticles. For both time-periods (24 h or 30 days), the relative microbial viability significantly decreased by increasing the CHX content (P < 0.001). Generally, the cell viability percentage of DPSCs exposed to MSN-PGA/Blank, CHX-loaded/MSN, and CHX-loaded/MSN-PGA, respectively was > 80% indicating low cytotoxicity profiles of experimental nanoparticles. After 9 months in artificial saliva (pH 7.4), the significantly highest micro-tensile bond strength value was recorded for 25:50 CHX/MSN and 25:50:50 CHX/MSN-PGA. A homogenous and widely distributed 50:50:50 CHX-loaded/MSN-PGA nanoparticles exhibited excellent bonding with the application of commercially available dentin adhesive.
Conclusions: A pH-sensitive CHX release response was noted when loaded in MSN grafted PGA nanoparticles. The formulated drug-loaded nanocarrier demonstrated excellent physicochemical, spectral, and biological characteristics. Showing considerable capacity to penetrate effectively inside dentinal tubules and having high antibacterial efficacy, this system could be potentially used in adhesive and restorative dentistry.

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PG #008/10402012 Faculty of Health and Medical Sciences; 00800/70237003 Dental School University of Western Australia; 00800/68001004 Colgate-Palmolive Company

Keywords: Adhesion; Antimicrobial; Chlorhexidine; Drug delivery; Mesoporous silica nanoparticle; pH-sensitive

0 (Anti-Bacterial Agents)
0 (Composite Resins)
0 (Dental Materials)
0 (Glycolates)
0WT12SX38S (glycolic acid)
7631-86-9 (Silicon Dioxide)
R4KO0DY52L (Chlorhexidine)

Date Created: 20210210 Date Completed: 20210914 Latest Revision: 20210914

20231215

PMC7871398

10.1186/s12951-021-00788-6

33563280