Epidermal growth factor/epidermal growth factor receptor moves into the osteoblasts' cell nuclei where it is involved to regulate STAT5's signaling.

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  • Additional Information
    • Source:
      Publisher: Ios Press Country of Publication: Netherlands NLM ID: 8807441 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8081 (Electronic) Linking ISSN: 09516433 NLM ISO Abbreviation: Biofactors Subsets: MEDLINE
    • Publication Information:
      Publication: Amsterdam : Ios Press
      Original Publication: Oxford ; Washington, DC : Published for the International Union of Biochemistry by IRL Press, [c1988-
    • Subject Terms:
      Cell Nucleus/*metabolism ; Epidermal Growth Factor/*metabolism ; ErbB Receptors/*metabolism ; Osteoblasts/*metabolism ; STAT5 Transcription Factor/*metabolism ; Signal Transduction/*physiology; Animals ; Cells, Cultured ; Enzyme-Linked Immunosorbent Assay ; Mice ; Microscopy, Confocal ; Models, Animal
    • Abstract:
      Epidermal growth factor (EGF) has vital biological impacts on the osteoblasts. However, the knowledge on the cellular properties of EGF/EGFR (epidermal growth factor receptor) on osteoblasts is scanty. As such, we explored the EGF/EGFR's cell behavior in the osteoblast (MC3T3-E1 cell) using the indirect immunofluorescence assay, Western-blot, and fluorescence resonance energy transfer. Our findings revealed that EGF could internalize into the cytoplasm under EGFR mediation. Besides, the co-localization analysis demonstrated that caveolin played a critical role in EGFR's endocytosis. We also analyzed the cytoplasmic trafficking pathway of EGF/EGFR in MC3T3-E1 cell. The colocalization analysis showed that EGFR entered into Rab5, Rab4, and Rab9-positive endosomes. More importantly, we found that EGFR could move into the MC3T3-E1 cells' nuclei. Based on this, we investigated the EGFR's nuclear-localized functions, and the results suggested that nuclear-localized EGFR has important biological functions. This work lays a foundation for further study on EGF/EGFR's biological functions on the osteoblasts.
      (© 2021 International Union of Biochemistry and Molecular Biology.)
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    • Grant Information:
      2018YFC1105603 National Key Research and Development Program of China; 20XD1402600 2020 Science and Technology Innovation Action Plan of Shanghai
    • Contributed Indexing:
      Keywords: EGF; EGFR; internalization; nuclear localization; osteoblasts; signaling pathway
    • Accession Number:
      0 (STAT5 Transcription Factor)
      62229-50-9 (Epidermal Growth Factor)
      EC 2.7.10.1 (ErbB Receptors)
    • Publication Date:
      Date Created: 20210127 Date Completed: 20211215 Latest Revision: 20211215
    • Publication Date:
      20250114
    • Accession Number:
      10.1002/biof.1709
    • Accession Number:
      33503297