Weighted gene co-expression network analysis identifies molecular pathways and hub genes involved in broiler White Striping and Wooden Breast myopathies.

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  • Additional Information
    • Source:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : Nature Publishing Group, copyright 2011-
    • Subject Terms:
      Collagen Type IV/*metabolism ; Extracellular Matrix/*physiology ; Muscular Diseases/*genetics ; Muscular Diseases/*pathology ; Pectoralis Muscles/*metabolism; Animals ; Chickens ; Collagen Type IV/genetics ; Gene Expression/genetics ; Gene Expression Profiling ; Gene Expression Regulation/genetics ; Meat/analysis ; Poultry Diseases/genetics ; Poultry Diseases/pathology ; Unfolded Protein Response/genetics
    • Abstract:
      In recent years, the poultry industry has experienced an increased incidence of myopathies affecting breasts of fast-growing broilers, such as White Striping (WS) and Wooden Breast (WB) defects. To explore the molecular mechanisms and genes involved in WS and WB onset, we decided to perform a Weighted Gene Co-expression Network Analysis (WGCNA) using the gene expression profile and meat quality parameters of Pectoralis major muscles analysed in our previous study. Among the 212 modules identified by WGCNA, the red, darkred, midnightblue and paleturquoise4 modules were chosen for subsequent analysis. Functional analysis evidenced pathways involved in extracellular matrix (ECM) organization, collagen metabolism, cellular signaling and unfolded protein response. The hub gene analysis showed several genes coding for ECM components as the most interconnected nodes in the gene network (e.g. COL4A1, COL4A2, LAMA2, LAMA4, FBLN5 and FBN1). In this regard, this study suggests that alterations in ECM composition could somehow activate the cascade of biological reactions that result in the growth-related myopathies onset, and the involvement of Collagen IV alterations in activating the endoplasmic reticulum (ER) stress response may be hypothesized. Therefore, our findings provide further and innovative knowledge concerning the molecular mechanisms related to the breast abnormalities occurrence in modern broilers.
    • References:
      Trends Endocrinol Metab. 2015 Jul;26(7):357-66. (PMID: 26059707)
      Nat Protoc. 2009;4(1):44-57. (PMID: 19131956)
      Essays Biochem. 2019 Sep 13;63(3):297-312. (PMID: 31387942)
      Sci Rep. 2019 Oct 17;9(1):14905. (PMID: 31624339)
      PLoS One. 2018 Dec 5;13(12):e0207346. (PMID: 30517117)
      Am J Hum Genet. 2019 May 2;104(5):847-860. (PMID: 31051113)
      Endocrinology. 2020 Feb 1;161(2):. (PMID: 31796960)
      Front Physiol. 2020 Jan 17;10:1581. (PMID: 32009982)
      Front Physiol. 2020 Feb 25;11:101. (PMID: 32158398)
      BMC Bioinformatics. 2008 Dec 29;9:559. (PMID: 19114008)
      Poult Sci. 2015 Jan;94(1):123-30. (PMID: 25577799)
      Am J Pathol. 2017 Mar;187(3):505-516. (PMID: 28056338)
      Animals (Basel). 2020 Jun 23;10(6):. (PMID: 32585889)
      Sci Rep. 2019 Oct 4;9(1):14324. (PMID: 31586140)
      BMC Syst Biol. 2014;8 Suppl 4:S11. (PMID: 25521941)
      Bioinformatics. 2009 Apr 15;25(8):1091-3. (PMID: 19237447)
      Vet Pathol. 2014 May;51(3):619-23. (PMID: 23892375)
      Essays Biochem. 2019 Sep 13;63(3):365-376. (PMID: 31395654)
      Front Physiol. 2020 May 08;11:343. (PMID: 32457639)
      Placenta. 2015 Aug;36(8):809-20. (PMID: 26059342)
      Genet Med. 2015 Nov;17(11):843-53. (PMID: 25719457)
      Avian Pathol. 2019 Feb;48(1):1-3. (PMID: 30246553)
      Poult Sci. 2017 Aug 1;96(8):3005-3009. (PMID: 28431094)
      Front Pharmacol. 2020 Feb 28;11:142. (PMID: 32184725)
      Meat Sci. 2000 Dec;56(4):397-402. (PMID: 22062170)
      Poult Sci. 2015 Dec;94(12):2870-9. (PMID: 26476091)
      Front Physiol. 2020 May 07;11:461. (PMID: 32457657)
      Compr Rev Food Sci Food Saf. 2019 Mar;18(2):565-583. (PMID: 33336940)
      Avian Pathol. 2017 Dec;46(6):623-643. (PMID: 28609139)
      Matrix Biol. 2018 Oct;71-72:188-204. (PMID: 29933045)
      Genome Res. 2003 Nov;13(11):2498-504. (PMID: 14597658)
      Front Physiol. 2020 Mar 13;11:169. (PMID: 32231585)
      Appl Clin Genet. 2019 Jul 03;12:113-130. (PMID: 31308722)
      BMC Genomics. 2018 Mar 20;19(1):202. (PMID: 29554873)
      Poult Sci. 2016 Nov 1;95(11):2724-2733. (PMID: 27450434)
      Avian Pathol. 2018 Feb;47(1):2-13. (PMID: 28714747)
      BMC Genomics. 2015 May 21;16:399. (PMID: 25994290)
      J Biol Chem. 2009 Dec 18;284(51):35872-84. (PMID: 19858219)
      Genes (Basel). 2019 Sep 25;10(10):. (PMID: 31557856)
      Front Physiol. 2020 Jun 16;11:580. (PMID: 32612536)
      BMC Genet. 2016 Apr 19;17:61. (PMID: 27094623)
      Hum Mol Genet. 2012 Oct 15;21(R1):R97-110. (PMID: 22914737)
      Mol Neurobiol. 2014 Feb;49(1):574-89. (PMID: 24014138)
      Nucleic Acids Res. 2000 Jan 1;28(1):27-30. (PMID: 10592173)
      J Clin Endocrinol Metab. 2014 May;99(5):1749-57. (PMID: 24601694)
      Antioxid Redox Signal. 2006 Sep-Oct;8(9-10):1391-418. (PMID: 16986999)
      Mol Genet Metab. 2012 Dec;107(4):635-47. (PMID: 22921888)
      Poult Sci. 2016 Dec 1;95(12):2771-2785. (PMID: 27591279)
      Gen Comp Endocrinol. 2020 Sep 1;295:113526. (PMID: 32540490)
    • Accession Number:
      0 (Collagen Type IV)
    • Publication Date:
      Date Created: 20210120 Date Completed: 20210914 Latest Revision: 20240330
    • Publication Date:
      20240330
    • Accession Number:
      PMC7815844
    • Accession Number:
      10.1038/s41598-021-81303-7
    • Accession Number:
      33469097