Microcapillary LAMP for rapid and sensitive detection of pathogen in bovine semen.

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  • Additional Information
    • Source:
      Publisher: Taylor & Francis Country of Publication: England NLM ID: 9011409 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2378 (Electronic) Linking ISSN: 10495398 NLM ISO Abbreviation: Anim Biotechnol Subsets: MEDLINE
    • Publication Information:
      Publication: 2005- : Abingdon, Oxford : Taylor & Francis
      Original Publication: New York, N.Y. : Marcel Dekker, c1990-
    • Subject Terms:
      Semen* ; Herpesvirus 1, Bovine*/genetics; Cattle ; Animals ; Nucleic Acid Amplification Techniques ; Real-Time Polymerase Chain Reaction/veterinary ; Sensitivity and Specificity
    • Abstract:
      A microcapillary-based loop-mediated isothermal amplification (µcLAMP) has been described for specific detection of infectious reproductive pathogens in semen samples of cattle without sophisticated instrumentation. Brucella abortus , Leptospira interrogans serovar Pomona and bovine herpesvirus 1 (BoHV-1) cultures were mixed in bovine semen samples. The µcLAMP assay is portable, user-friendly, cost-effective, and suitable to be performed as a POC diagnostic test. We have demonstrated high sensitivity and specificity of µcLAMP for detection of Brucella , Leptospira, and BoHV-1 in bovine semen samples comparable to PCR and qPCR assays. Thus, µcLAMP would be a promising field-based test for monitoring various infectious pathogens in biological samples.HighlightsDetect infectious organism in bovines semenReduction in carryover contamination is an important attribute, which may reduce the false-positive reaction.µcLAMP is a miniaturized form, which could be performed with a minimum volume of reagents.The µcLAMP assay is portable, user-friendly, and suitable to be performed as a POC diagnostic test.
    • Contributed Indexing:
      Keywords: BoHV-1; Brucella; LAMP; Leptospira; Point-Of-Care (POC); microcapillary
    • Subject Terms:
      LAMP assay
    • Publication Date:
      Date Created: 20210111 Date Completed: 20221121 Latest Revision: 20221121
    • Publication Date:
      20221213
    • Accession Number:
      10.1080/10495398.2020.1863225
    • Accession Number:
      33427030