A Murine Model for Chronic A. fumigatus Airway Infections.

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  • Additional Information
    • Source:
      Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
    • Publication Information:
      Publication: Totowa, NJ : Humana Press
      Original Publication: Clifton, N.J. : Humana Press,
    • Subject Terms:
      Aspergillus fumigatus/*pathogenicity ; Lung/*microbiology ; Pulmonary Aspergillosis/*microbiology; Adaptive Immunity ; Animals ; Antifungal Agents/pharmacology ; Aspergillus fumigatus/drug effects ; Aspergillus fumigatus/immunology ; Chronic Disease ; Disease Models, Animal ; Host-Pathogen Interactions ; Immunity, Innate ; Lung/drug effects ; Lung/immunology ; Lung/pathology ; Pulmonary Aspergillosis/drug therapy ; Pulmonary Aspergillosis/immunology ; Pulmonary Aspergillosis/pathology ; Time Factors
    • Abstract:
      In addition to causing acute invasive infections in immunocompromised patients, the mold Aspergillus fumigatus causes chronic infections in patients with chronic pulmonary conditions such as cystic fibrosis. Here we describe a non-lethal model of chronic pulmonary aspergillosis in which immunocompetent mice are endotracheally infected with A. fumigatus conidia embedded in agar beads. This approach results in the establishment of hyphal infection within the airways of mice for up to a 28-day period and is amenable to the study of innate and adaptive antifungal responses, fungal mutant strains, and antifungal agents.
    • References:
      Rapaka RR, Kolls JK (2009) Pathogenesis of allergic bronchopulmonary aspergillosis in cystic fibrosis: current understanding and future directions. Med Mycol 47(Suppl 1):S331–S337. https://doi.org/10.1080/13693780802266777. (PMID: 10.1080/1369378080226677718668399)
      Stevens DA, Moss RB, Kurup VP, Knutsen AP, Greenberger P, Judson MA et al (2003) Allergic bronchopulmonary aspergillosis in cystic fibrosis--state of the art: Cystic Fibrosis Foundation consensus conference. Clin Infect Dis 37(Suppl 3):S225–S264. https://doi.org/10.1086/376525. (PMID: 10.1086/37652512975753)
      Clemons KV, Stevens DA (2005) The contribution of animal models of aspergillosis to understanding pathogenesis, therapy and virulence. Med Mycol 43(Suppl 1):S101–S110. (PMID: 10.1080/13693780500051919)
      Park SJ, Mehrad B (2009) Innate immunity to Aspergillus species. Clin Microbiol Rev 22(4):535–551. https://doi.org/10.1128/CMR.00014-09. (PMID: 10.1128/CMR.00014-09198228872772361)
      Sheppard DC, Rieg G, Chiang LY, Filler SG, Edwards JE Jr, Ibrahim AS (2004) Novel inhalational murine model of invasive pulmonary aspergillosis. Antimicrob Agents Chemother 48(5):1908–1911. https://doi.org/10.1128/AAC.48.5.1908-1911.2004. (PMID: 10.1128/AAC.48.5.1908-1911.200415105158400587)
      Urb M, Snarr BD, Wojewodka G, Lehoux M, Lee MJ, Ralph B et al (2015) Evolution of the immune response to chronic airway colonization with aspergillus fumigatus hyphae. Infect Immun 83(9):3590–3600. https://doi.org/10.1128/IAI.00359-15. (PMID: 10.1128/IAI.00359-15261238034534667)
    • Contributed Indexing:
      Keywords: Agar bead model; Aspergillus fumigatus; Chronic airway infection; Immunocompetent; Mouse model
    • Accession Number:
      0 (Antifungal Agents)
    • Publication Date:
      Date Created: 20210106 Date Completed: 20210331 Latest Revision: 20210331
    • Publication Date:
      20250114
    • Accession Number:
      10.1007/978-1-0716-1182-1_15
    • Accession Number:
      33405041