GDF-5 induces epidermal stem cell migration via RhoA-MMP9 signalling.

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  • Additional Information
    • Source:
      Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 101083777 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1582-4934 (Electronic) Linking ISSN: 15821838 NLM ISO Abbreviation: J Cell Mol Med Subsets: MEDLINE
    • Publication Information:
      Publication: Oxford, England : Wiley-Blackwell
      Original Publication: Bucharest : "Carol Davila" University Press, 2000-
    • Subject Terms:
      Cell Movement/*genetics ; Epidermal Cells/*metabolism ; Growth Differentiation Factor 5/*metabolism ; Matrix Metalloproteinase 9/*metabolism ; Stem Cells/*metabolism ; rhoA GTP-Binding Protein/*metabolism; Animals ; Cells, Cultured ; Gene Expression ; Gene Expression Regulation ; Matrix Metalloproteinase 9/genetics ; Mice ; RNA, Messenger/genetics
    • Abstract:
      The migration of epidermal stem cells (EpSCs) is critical for wound re-epithelization and wound healing. Recently, growth/differentiation factor-5 (GDF-5) was discovered to have multiple biological effects on wound healing; however, its role in EpSCs remains unclear. In this work, recombinant mouse GDF-5 (rmGDF-5) was found via live imaging in vitro to facilitate the migration of mouse EpSCs in a wound-scratch model. Western blot and real-time PCR assays demonstrated that the expression levels of RhoA and matrix metalloproteinase-9 (MMP9) were correlated with rmGDF-5 concentration. Furthermore, we found that rmGDF-5 stimulated mouse EpSC migration in vitro by regulating MMP9 expression at the mRNA and protein levels through the RhoA signalling pathway. Moreover, in a deep partial-thickness scald mouse model in vivo, GDF-5 was confirmed to promote EpSC migration and MMP9 expression via RhoA, as evidenced by the tracking of cells labelled with 5-bromo-2-deoxyuridine (BrdU). The current study showed that rmGDF-5 can promote mouse EpSC migration in vitro and in vivo and that GDF-5 can trigger the migration of EpSCs via RhoA-MMP9 signalling.
      (© 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.)
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    • Grant Information:
      2018M633760 the Postdoctoral Science Foundation of China; 81701925 the National Natural Science Foundation of China; cstc2019jcyj-cxttX0001 the Natural Science Foundation Innovation Group Science Foundation of Chongqing
    • Contributed Indexing:
      Keywords: cell migration; epidermal stem cells; growth/differentiation factor-5; matrix metalloproteinases
    • Accession Number:
      0 (Gdf5 protein, mouse)
      0 (Growth Differentiation Factor 5)
      0 (RNA, Messenger)
      EC 3.4.24.35 (Matrix Metalloproteinase 9)
      EC 3.6.5.2 (RhoA protein, mouse)
      EC 3.6.5.2 (rhoA GTP-Binding Protein)
    • Publication Date:
      Date Created: 20201228 Date Completed: 20210923 Latest Revision: 20210923
    • Publication Date:
      20231215
    • Accession Number:
      PMC7882973
    • Accession Number:
      10.1111/jcmm.15925
    • Accession Number:
      33369147