Sedimentation Velocity Methods for the Characterization of Protein Heterogeneity and Protein Affinity Interactions.

Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE

Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,

Biophysical Phenomena* ; Biosensing Techniques*; Proteins/*chemistry; Algorithms ; Data Analysis ; Models, Theoretical ; Protein Binding ; Temperature ; Ultracentrifugation/methods

Sedimentation velocity analytical ultracentrifugation is a powerful and versatile tool for the characterization of proteins and macromolecular complexes in solution. The direct modeling of the sedimentation process using modern computational strategies allows among others to assess the homogeneity/heterogeneity state of protein samples and to characterize protein associations. In this chapter, we will provide theoretical backgrounds and protocols to analyze the size distribution of protein samples and to determine the affinity of protein-protein hetero-associations.

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Keywords: Analytical ultracentrifugation; Hetero-association; Protein heterogeneity; Protein interaction; Sedimentation coefficient; Sedimentation velocity

0 (Proteins)

Date Created: 20201210 Date Completed: 20210331 Latest Revision: 20210331

20221213

10.1007/978-1-0716-1126-5_9

33301117