Hypermucoviscous/hypervirulent and extensively drug-resistant QnrB2-, QnrS1-, and CTX-M-3-coproducing Klebsiella pneumoniae ST2121 isolated from an infected elephant (Loxodonta africana).

Publisher: Elsevier Scientific Pub. Co Country of Publication: Netherlands NLM ID: 7705469 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2542 (Electronic) Linking ISSN: 03781135 NLM ISO Abbreviation: Vet Microbiol Subsets: MEDLINE

Original Publication: Amsterdam, Elsevier Scientific Pub. Co.

Drug Resistance, Multiple, Bacterial*; Anti-Bacterial Agents/*pharmacology ; Elephants/*microbiology ; Klebsiella Infections/*veterinary ; Klebsiella pneumoniae/*isolation & purification ; Klebsiella pneumoniae/*pathogenicity ; beta-Lactamases/*biosynthesis; Animals ; Animals, Zoo/microbiology ; Bacterial Typing Techniques ; Brazil ; Escherichia coli/genetics ; Klebsiella Infections/microbiology ; Klebsiella pneumoniae/drug effects ; Klebsiella pneumoniae/genetics ; Larva/microbiology ; Microbial Sensitivity Tests ; Moths/microbiology ; Multilocus Sequence Typing ; Plasmids/genetics ; Quinolones/pharmacology ; Virulence ; beta-Lactamases/genetics ; beta-Lactams/pharmacology

The rapid dissemination of extended-spectrum β-lactamases (ESBLs)-producing Enterobacterales from different spheres worldwide over recent years has become a serious problem in both human and veterinary medicine. CTX-M-3-type ESBL has only been reported on few occasions, and in Brazil the bla CTX-M-3 gene has been identified only once in clinical strains. In this study, we aimed to molecularly characterize a hypermucoviscous (hm), hypervirulent (hv), and extensively drug-resistant (XDR) Klebsiella pneumoniae strain isolated from a lung tissue culture of an infected elephant. The A246 strain belonged to ST2121 and presented hm phenotype, hypervirulence-associated genes, and carried bla CTX-M-3 and plasmid-mediated quinolone resistance genes (qnrB2 and qnrS1) on an IncFII-IncQ1-IncM1 multireplicon plasmid (pA246-CTX-M-3, ∼ 162 kb). A novel genetic context of bla CTX-M-3, in which a 482-bp ISEcp1 was truncated by an IS26, was also harbored by pA246-CTX-M-3. Furthermore, in vivo experiments revealed that the hm/hv A246 strain killed 100 % of the Galleria mellonella larvae at 72 h post-infection. Our findings evidence the intercontinental dissemination of a rare K. pneumoniae ST2121 and the multidrug resistance IncFII-IncQ1-IncM1 plasmid. Therefore, to the best of our knowledge, this is the first report of an XDR K. pneumoniae coproducing CTX-M-3, QnrB2, and QnrS1 isolated from captive wild animals.
(Copyright © 2020 Elsevier B.V. All rights reserved.)

Keywords: CTX-M-3; Extensively drug-resistant; Hypermucoviscous; Hypervirulent; Klebsiella pneumoniae

0 (Anti-Bacterial Agents)
0 (Quinolones)
0 (beta-Lactams)
EC 3.5.2.- (beta-lactamase CTX-M-3)
EC 3.5.2.6 (beta-Lactamases)

Date Created: 20201111 Date Completed: 20210715 Latest Revision: 20210715

20240829

10.1016/j.vetmic.2020.108909

33176213