1-Methylpyrene induces chromosome loss and mitotic apparatus damage in a Chinese hamster V79-derived cell line expressing both human CYP1A2 and sulfotransferase 1A1.

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  • Author(s): Yu H;Yu H; Li Z; Li Z; Yang Z; Yang Z; Song M; Song M; Liu Y; Liu Y
  • Source:
    Chemico-biological interactions [Chem Biol Interact] 2020 Dec 01; Vol. 332, pp. 109283. Date of Electronic Publication: 2020 Oct 06.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Ireland NLM ID: 0227276 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7786 (Electronic) Linking ISSN: 00092797 NLM ISO Abbreviation: Chem Biol Interact Subsets: MEDLINE
    • Publication Information:
      Publication: Limerick : Elsevier
      Original Publication: Amsterdam, Elsevier.
    • Subject Terms:
      Arylsulfotransferase/*metabolism ; Chromosomes, Mammalian/*metabolism ; Cytochrome P-450 CYP1A2/*metabolism ; Mitosis/*drug effects ; Pyrenes/*pharmacology; Animals ; Cell Line ; Cell Shape/drug effects ; Cell Survival/drug effects ; Centromere Protein B/metabolism ; Cricetinae ; Humans ; Micronucleus, Germline/drug effects ; Micronucleus, Germline/metabolism ; Mitotic Index ; Spindle Apparatus/drug effects ; Spindle Apparatus/metabolism
    • Abstract:
      1-Methylpyrene (1-MP) is a ubiquitous environmental pollutant and rodent carcinogen. Its mutagenic activity depends on sequential activation by various CYP and sulfotransferase (SULT) enzymes. Previously we have observed induction of micronuclei and mitotic arrest by 1-MP in a Chinese hamster (V79)-derived cell line expressing both human CYP1A2 and SULT1A1 (V79-hCYP1A2-hSULT1A1), however, the mode of chromosome damage and the involvement of mitotic tubulin structures have not been clarified. In this study, we used immunofluorescent staining of centromere protein B (CENP-B) with the formed micronuclei, and that of β- and γ-tubulin reflecting the structures of mitotic spindle and centrioles, respectively, in V79-hCYP1A2-hSULT1A1 cells. The results indicated that 1-MP induced micronuclei in V79-hCYP1A2-hSULT1A1 cells from 0.125 to 2 μM under a 24 h/0 h (exposure/recovery) regime, while in the parental V79-Mz cells micronuclei were induced by 1-MP only at concentrations ≥ 8 μM; in both cases, the micronuclei induced by 1-MP were predominantly CENP-B positive. Following 54 h of exposure, 1-MP induced mitotic spindle non-congression and centrosome amplification (multipolar mitosis) in V79-hCYP1A2-hSULT1A1 cells, and anaphase/telophase retardation, at concentrations ≥ 0.125 μM with concentration-dependence; while in V79-Mz cells it was inactive up to 8 μM. This study suggests that in mammalian cells proficient in activating enzymes 1-MP may induce chromosome loss and mitotic disturbance, probably by interfering with the mitotic spindle and centrioles.
      (Copyright © 2020 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: 1-Methylpyrene; Centrioles; Centromere protein B; Metabolic activation; Micronuclei; Mitotic spindle
    • Accession Number:
      0 (Centromere Protein B)
      0 (Pyrenes)
      AB8420OXQA (1-methylpyrene)
      EC 1.14.14.1 (CYP1A2 protein, human)
      EC 1.14.14.1 (Cytochrome P-450 CYP1A2)
      EC 2.8.2.1 (Arylsulfotransferase)
      EC 2.8.2.1 (SULT1A1 protein, human)
    • Publication Date:
      Date Created: 20201009 Date Completed: 20201211 Latest Revision: 20201214
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.cbi.2020.109283
    • Accession Number:
      33035519