Correlative Light and Scanning Electron Microscopy to Study Interactions of Salmonella enterica with Polarized Epithelial Cell Monolayers.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Kommnick C;Kommnick C; Hensel M; Hensel M; Hensel M
  • Source:
    Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2182, pp. 103-115.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
    • Publication Information:
      Publication: Totowa, NJ : Humana Press
      Original Publication: Clifton, N.J. : Humana Press,
    • Subject Terms:
      Cell Polarity/*physiology ; Epithelial Cells/*microbiology ; Microscopy, Electron, Scanning/*methods ; Salmonella Infections/*microbiology ; Salmonella enterica/*pathogenicity; Animals ; Cell Line ; Dogs ; Madin Darby Canine Kidney Cells
    • Abstract:
      Live cell fluorescence imaging is the method of choice to visualize dynamic cellular processes in time and space, such as adhesion to and invasion of polarized epithelial cells by Salmonella enterica sv. Typhimurium. Scanning electron microscopy provides highest resolution of surface structures of infected cells, providing ultrastructure of the apical side of host cells and infecting Salmonella. Combining both methods toward correlative light and scanning electron microscopy (CLSEM) enables new insights in adhesion and invasion mechanisms regarding dynamics over time, and high spatial resolution with precise time lines. To correlate fast live cell imaging of polarized monolayer cells with scanning electron microscopy, we developed a robust method by using gold mesh grids as convenient CLSEM carriers for standard microscopes. By this, we were able to unravel the morphology of the apical structures of monolayers of polarized epithelial cells at distinct time points during Salmonella infection.
    • Contributed Indexing:
      Keywords: Adhesion; CLEM; CLSEM; Correlative light and electron microscopy; Correlative light and scanning electron microscopy; Invasion; LCI; Live cell imaging; Mesh grid; Monolayer; Morphometric analysis; Polarized epithelial cells; SEM; Salmonella enterica serovar Typhimurium; Scanning electron microscopy; Spinning disc confocal microscopy
    • Publication Date:
      Date Created: 20200907 Date Completed: 20210318 Latest Revision: 20210318
    • Publication Date:
      20240829
    • Accession Number:
      10.1007/978-1-0716-0791-6_10
    • Accession Number:
      32894490