Preselection TCR repertoire predicts CD4 + and CD8 + T-cell differentiation state.

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  • Additional Information
    • Source:
      Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 0374672 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2567 (Electronic) Linking ISSN: 00192805 NLM ISO Abbreviation: Immunology Subsets: MEDLINE
    • Publication Information:
      Original Publication: Oxford : Blackwell Scientific Publications
    • Subject Terms:
      CD4-Positive T-Lymphocytes/*immunology ; CD8-Positive T-Lymphocytes/*immunology ; Genes, T-Cell Receptor beta/*genetics ; Receptors, Antigen, T-Cell, alpha-beta/*genetics ; T-Lymphocyte Subsets/*immunology; Cell Differentiation ; Cells, Cultured ; Clonal Selection, Antigen-Mediated ; Female ; High-Throughput Nucleotide Sequencing ; Humans ; Lymphocyte Activation ; Male ; Middle Aged ; V(D)J Recombination
    • Abstract:
      T cells must display diversity regarding both the cell state and T-cell receptor (TCR) repertoire to provide effective immunity against pathogens; however, the generation and evolution of cellular T-cell heterogeneity in the adaptive immune system remains unclear. In the present study, a combination of multiplex PCR and immune repertoire sequencing (IR-seq) was used for a standardized analysis of the TCR β-chain repertoire of CD4 + naive, CD4 + memory, CD8 + naive and CD8 + memory T cells. We showed that the T-cell subsets could be distinguished from each another with regard to the TCR β-chain (TCR-β) diversity, CDR3 length distribution and TRBV usage, which could be observed both in the preselection and in the post-selection repertoire. Moreover, the Dβ-Jβ and Vβ-Dβ combination patterns at the initial recombination step, template-independent insertion of nucleotides and inter-subset overlap were consistent between the pre- and post-selection repertoires, with a remarkably positive correlation. Taken together, these results support differentiation of the CD4 + and CD8 + T-cell subsets prior to thymic selection, and these differences survived both positive and negative selection. In conclusion, these findings provide deeper insight into the generation and evolution of TCR repertoire generation.
      (© 2020 John Wiley & Sons Ltd.)
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    • Grant Information:
      2019GXNSFBA245032 Natural Science Foundation of Guangxi Province; 20190218-5-5 Guilin science research and technology development project; 2019C04005 Key Research & Developement Plan of Zhejiang Province; 2019KF004 the open funds of the Guangxi Key Laboratory of Tumor Immunology and Microenvironmental Regulation; 2018ZX10301401-005 the major national S&T projects for infectious diseases; 81571953 National Natural Science Foundation of China; 2018YFC2000500-004 National Key Science and Technology Project
    • Contributed Indexing:
      Keywords: T-cell receptor; cell subsets; deep sequencing
    • Accession Number:
      0 (Receptors, Antigen, T-Cell, alpha-beta)
    • Publication Date:
      Date Created: 20200903 Date Completed: 20210526 Latest Revision: 20211203
    • Publication Date:
      20221213
    • Accession Number:
      PMC7692249
    • Accession Number:
      10.1111/imm.13256
    • Accession Number:
      32875554