Vitrification for cryopreservation of 2D and 3D stem cells culture using high concentration of cryoprotective agents.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 101088663 Publication Model: Electronic Cited Medium: Internet ISSN: 1472-6750 (Electronic) Linking ISSN: 14726750 NLM ISO Abbreviation: BMC Biotechnol Subsets: MEDLINE
    • Publication Information:
      Original Publication: [London] : BioMed Central, 2001-
    • Subject Terms:
      Vitrification*; Cell Culture Techniques/*methods ; Cryopreservation/*methods ; Cryoprotective Agents/*chemistry ; Stem Cells/*cytology; Cell Proliferation ; Cell Survival ; Freezing ; Humans ; Mesenchymal Stem Cells ; Reactive Oxygen Species
    • Abstract:
      Background: Vitrification is the most promising technology for successful cryopreservation of living organisms without ice crystal formation. However, high concentrations (up to ~ 6-8 M) of cryoprotective agents (CPAs) used in stem cell induce osmotic and metabolic injuries. Moreover, the application of conventional slow-freezing methods to cultures of 3-D organoids of stem cells in various studies, is limited by their size.
      Results: In this study, we evaluated the effect of high concentrations of CPAs including cytotoxicity and characterized human mesenchymal stem cell (MSC) at single cell level. The cell viability, cellular damage, and apoptotic mechanisms as well as the proliferation capacity and multipotency of cells subjected to vitrification were similar to those in the slow-freezing group. Furthermore, we identified the possibility of vitrification of size-controlled 3-D spheroids for cryopreservation of organoid with high survivability.
      Conclusions: Our results demonstrate successful vitrification of both single cell and spheroid using high concentration of CPAs in vitro without cytotoxicity.
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    • Grant Information:
      NRF-2017R1D1A1B03032681 International Korea Research Institute of Bioscience and Biotechnology; NRF-2018R1C1B6007354 International Konkuk University; UD170032ID International Korea Agency for Defense Development
    • Contributed Indexing:
      Keywords: Cryoprotective agent; In vitro; MSC; Spheroid; Vitrification
    • Accession Number:
      0 (Cryoprotective Agents)
      0 (Reactive Oxygen Species)
    • Publication Date:
      Date Created: 20200827 Date Completed: 20210614 Latest Revision: 20210614
    • Publication Date:
      20221213
    • Accession Number:
      PMC7449025
    • Accession Number:
      10.1186/s12896-020-00636-9
    • Accession Number:
      32843026