Peptide Release after Simulated Infant In Vitro Digestion of Dry Heated Cow's Milk Protein and Transport of Potentially Immunoreactive Peptides across the Caco-2 Cell Monolayer.

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  • Additional Information
    • Source:
      Publisher: MDPI Publishing Country of Publication: Switzerland NLM ID: 101521595 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6643 (Electronic) Linking ISSN: 20726643 NLM ISO Abbreviation: Nutrients Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI Publishing
    • Subject Terms:
      Hot Temperature* ; Milk* ; Protein Transport* ; Proteolysis*; Caco-2 Cells/*metabolism ; Digestion/*physiology ; Food Handling/*methods ; Infant Nutritional Physiological Phenomena/*physiology ; Intestines/*physiology ; Milk Hypersensitivity/*prevention & control ; Milk Proteins/*metabolism ; Peptides/*metabolism; Animals ; Epitopes, T-Lymphocyte ; Humans ; Immunoglobulin E ; In Vitro Techniques ; Infant ; Milk Proteins/chemistry ; Milk Proteins/immunology
    • Abstract:
      Dry heating of cow's milk protein, as applied in the production of "baked milk", facilitates the resolution of cow's milk allergy symptoms upon digestion. The heating and glycation-induced changes of the protein structure can affect both digestibility and immunoreactivity. The immunological consequences may be due to changes in the peptide profile of the digested dry heated milk protein. Therefore, cow's milk protein powder was heated at low temperature (60 °C) and high temperature (130 °C) and applied to simulated infant in vitro digestion. Digestion-derived peptides after 10 min and 60 min in the intestinal phase were measured using LC-MS/MS. Moreover, digests after 10 min intestinal digestion were applied to a Caco-2 cell monolayer. T-cell epitopes were analysed using prediction software, while specific immunoglobin E (sIgE) binding epitopes were identified based on the existing literature. The largest number of sIgE binding epitopes was found in unheated samples, while T-cell epitopes were equally represented in all samples. Transport of glycated peptide indicated a preference for glucosyl lysine and lactosyl-lysine-modified peptides, while transport of peptides containing epitope structures was limited. This showed that the release of immunoreactive peptides can be affected by the applied heating conditions; however, availability of peptides containing epitopes might be limited.
    • References:
      J Allergy Clin Immunol. 1998 May;101(5):660-71. (PMID: 9600504)
      J Med Chem. 2012 Jun 28;55(12):5859-67. (PMID: 22680612)
      Clin Exp Allergy. 2018 Oct;48(10):1345-1353. (PMID: 29974988)
      J Agric Food Chem. 2017 Jul 19;65(28):5778-5788. (PMID: 28653535)
      Nutrients. 2019 Nov 11;11(11):. (PMID: 31718010)
      Front Nutr. 2019 Mar 12;6:25. (PMID: 30931311)
      Nat Biotechnol. 2008 Dec;26(12):1367-72. (PMID: 19029910)
      Crit Rev Ther Drug Carrier Syst. 1988;4(3):197-220. (PMID: 3276405)
      FEBS Lett. 2000 Feb 11;467(2-3):239-44. (PMID: 10675546)
      J Biol Chem. 2014 Mar 14;289(11):7919-28. (PMID: 24505139)
      Food Funct. 2017 Oct 18;8(10):3769-3782. (PMID: 28959809)
      Biophys Chem. 2005 Jan 1;113(1):83-91. (PMID: 15617813)
      Food Chem. 2017 Mar 15;219:477-489. (PMID: 27765254)
      J Allergy Clin Immunol. 2010 Jan;125(1):175-83.e1-11. (PMID: 19864011)
      Chembiochem. 2011 May 16;12(8):1270-9. (PMID: 21538757)
      Molecules. 2020 Mar 12;25(6):. (PMID: 32178391)
      Mol Immunol. 2004 Nov;41(12):1225-34. (PMID: 15482858)
      Food Res Int. 2019 Apr;118:32-39. (PMID: 30898349)
      Structure. 2011 May 11;19(5):722-32. (PMID: 21565706)
      Mol Nutr Food Res. 2010 Nov;54(11):1677-89. (PMID: 20521278)
      Amino Acids. 2012 Apr;42(4):1111-8. (PMID: 20953645)
      Food Chem. 2018 Feb 1;240:338-345. (PMID: 28946281)
      Clin Exp Allergy. 2006 Mar;36(3):303-10. (PMID: 16499640)
      Food Funct. 2020 Jun 24;11(6):5240-5256. (PMID: 32458959)
      J Allergy Clin Immunol. 2011 Jul;128(1):125-131.e2. (PMID: 21601913)
      Nutrients. 2017 Aug 04;9(8):. (PMID: 28777346)
      J Agric Food Chem. 2015 Aug 5;63(30):6787-98. (PMID: 26161498)
      J Agric Food Chem. 2009 Jun 10;57(11):4974-82. (PMID: 19489627)
      J Proteomics. 2016 Feb 16;134:112-126. (PMID: 26210590)
      Peptides. 2017 Feb;88:18-31. (PMID: 27979737)
      Nucleic Acids Res. 1989 Aug 25;17(16):6739. (PMID: 2701948)
      Nutrients. 2019 Jun 25;11(6):. (PMID: 31242665)
      Food Funct. 2020 Jan 29;11(1):358-369. (PMID: 31799529)
      Biochem Soc Trans. 2003 Dec;31(Pt 6):1383-5. (PMID: 14641068)
      J Sci Food Agric. 2019 Apr;99(6):3069-3077. (PMID: 30511448)
      Allergol Int. 2015 Oct;64(4):332-43. (PMID: 26433529)
      Food Funct. 2013 Jul;4(7):1032-9. (PMID: 23435675)
      Chem Sci. 2016 Apr 21;7(4):2492-2500. (PMID: 28660018)
      J Agric Food Chem. 2004 Mar 10;52(5):1398-403. (PMID: 14995152)
      J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Feb 1;878(3-4):295-308. (PMID: 19962948)
      Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7114-8. (PMID: 8041755)
      Int J Mol Sci. 2020 Jun 26;21(12):. (PMID: 32604964)
      Nat Commun. 2017 Sep 5;8(1):438. (PMID: 28874712)
      Curr Opin Allergy Clin Immunol. 2009 Jun;9(3):234-7. (PMID: 19444093)
      Mol Nutr Food Res. 2014 Jan;58(1):194-205. (PMID: 24395537)
    • Grant Information:
      14536 Nederlandse Organisatie voor Wetenschappelijk Onderzoek
    • Contributed Indexing:
      Keywords: Caco-2 cell; allergenicity; cow’s milk protein; glycation; immunogenicity; peptides
    • Accession Number:
      0 (Epitopes, T-Lymphocyte)
      0 (Milk Proteins)
      0 (Peptides)
      37341-29-0 (Immunoglobulin E)
    • Publication Date:
      Date Created: 20200823 Date Completed: 20210326 Latest Revision: 20210326
    • Publication Date:
      20221213
    • Accession Number:
      PMC7468992
    • Accession Number:
      10.3390/nu12082483
    • Accession Number:
      32824739