Dynamic Measurement and Imaging of Capillaries, Arterioles, and Pericytes in Mouse Heart.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Zhao G;Zhao G;Zhao G; Joca HC; Joca HC; Lederer WJ; Lederer WJ
  • Source:
    Journal of visualized experiments : JoVE [J Vis Exp] 2020 Jul 29 (161). Date of Electronic Publication: 2020 Jul 29.
  • Publication Type:
    Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Video-Audio Media
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: MYJoVE Corporation Country of Publication: United States NLM ID: 101313252 Publication Model: Electronic Cited Medium: Internet ISSN: 1940-087X (Electronic) Linking ISSN: 1940087X NLM ISO Abbreviation: J Vis Exp Subsets: MEDLINE
    • Publication Information:
      Original Publication: [Boston, Mass. : MYJoVE Corporation, 2006]-
    • Subject Terms:
      Imaging, Three-Dimensional*; Arterioles/*diagnostic imaging ; Capillaries/*diagnostic imaging ; Pericytes/*physiology; Animals ; Arterioles/drug effects ; Arterioles/physiology ; Capillaries/drug effects ; Capillaries/physiology ; Catheterization ; Fluorescent Dyes/metabolism ; Hemodynamics/drug effects ; Mice, Inbred C57BL ; Perfusion ; Pericytes/drug effects ; Pinacidil/pharmacology ; Pressure ; Regional Blood Flow/drug effects ; Regional Blood Flow/physiology ; Vasoconstriction/drug effects ; Vasodilation/drug effects ; Vasodilation/physiology ; Wheat Germ Agglutinins/metabolism
    • Abstract:
      Coronary arterial tone along with the opening or closing of the capillaries largely determine the blood flow to cardiomyocytes at constant perfusion pressure. However, it is difficult to monitor the dynamic changes of the coronary arterioles and the capillaries in the whole heart, primarily due to its motion and non-stop beating. Here we describe a method that enables monitoring of arterial perfusion rate, pressure and the diameter changes of the arterioles and capillaries in mouse right ventricular papillary muscles. The mouse septal artery is cannulated and perfused at a constant flow or pressure with the other dynamically measured. After perfusion with a fluorescently labeled lectin (e.g., Alexa Fluor-488 or -633 labeled Wheat-Germ Agglutinin, WGA), the arterioles and capillaries (and other vessels) in right ventricle papillary muscle and septum could be readily imaged. The vessel-diameter changes could then be measured in the presence or absence of heart contractions. When genetically encoded fluorescent proteins were expressed, specific features could be monitored. For examples, pericytes were visualized in mouse hearts that expressed NG2-DsRed. This method has provided a useful platform to study the physiological functions of capillary pericytes in heart. It is also suitable for studying the effect of reagents on the blood flow in heart by measuring the vascular/capillary diameter and the arterial luminal pressure simultaneously. This preparation, combined with a state-of-the-art optic imaging system, allows one to study the blood flow and its control at cellular and molecular level in the heart under near-physiological conditions.
    • Accession Number:
      0 (Fluorescent Dyes)
      0 (Wheat Germ Agglutinins)
      7B0ZZH8P2W (Pinacidil)
    • Publication Date:
      Date Created: 20200818 Date Completed: 20201030 Latest Revision: 20201030
    • Publication Date:
      20231215
    • Accession Number:
      10.3791/61566
    • Accession Number:
      32804163