Selective Chemical Labeling and Sequencing of 5-Carboxylcytosine in DNA at Single-Base Resolution.

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  • Additional Information
    • Source:
      Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, American Chemical Society.
    • Subject Terms:
      Cytosine/*analogs & derivatives ; DNA, Neoplasm/*chemistry ; Molecular Probes/*chemistry; Chromatography, Liquid ; Cytosine/analysis ; DNA, Neoplasm/genetics ; DNA, Neoplasm/isolation & purification ; HeLa Cells ; Humans ; Mass Spectrometry ; Molecular Probes/chemical synthesis ; Molecular Structure ; Morpholines/chemistry ; Polymerase Chain Reaction
    • Abstract:
      5-Carboxylcytosine (5caC) plays a vital role in the dynamics of DNA demethylation, and sequencing of its sites will help us dig out more biological functions of 5caC. Herein, we present a novel chemical method to efficiently label 5caC distinguished from other bases in DNA. Combined with bisulfite sequencing, 5caC sites can be located at single-base resolution, and the efficiency of 5caC labeling is 92% based on the Sanger sequencing data. Furthermore, dot blot assays have confirmed that 5caC-containing DNA isolated from HeLa cells was successfully labeled using our method. We expect that our strategy can be further applied to selectively tagging other carboxyl-modified bases and mapping their sites in RNA.
    • Accession Number:
      0 (4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride)
      0 (5-carboxylcytosine)
      0 (DNA, Neoplasm)
      0 (Molecular Probes)
      0 (Morpholines)
      8J337D1HZY (Cytosine)
    • Publication Date:
      Date Created: 20200818 Date Completed: 20210216 Latest Revision: 20210216
    • Publication Date:
      20250114
    • Accession Number:
      10.1021/acs.analchem.0c03201
    • Accession Number:
      32803958