Cell-cell adhesion in plant grafting is facilitated by β-1,4-glucanases.

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  • Additional Information
    • Source:
      Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 0404511 Publication Model: Print Cited Medium: Internet ISSN: 1095-9203 (Electronic) Linking ISSN: 00368075 NLM ISO Abbreviation: Science Subsets: MEDLINE
    • Publication Information:
      Publication: : Washington, DC : American Association for the Advancement of Science
      Original Publication: New York, N.Y. : [s.n.] 1880-
    • Subject Terms:
      Cell Adhesion/*physiology ; Cell Communication/*physiology ; Cellulase/*metabolism ; Horticulture/*methods ; Plant Proteins/*metabolism ; Nicotiana/*physiology; Cell Adhesion/genetics ; Cell Communication/genetics ; Cellulase/genetics ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Plant Proteins/genetics ; Nicotiana/enzymology ; Nicotiana/genetics ; Transcription, Genetic
    • Abstract:
      Plant grafting is conducted for fruit and vegetable propagation, whereby a piece of living tissue is attached to another through cell-cell adhesion. However, graft compatibility limits combinations to closely related species, and the mechanism is poorly understood. We found that Nicotiana is capable of graft adhesion with a diverse range of angiosperms. Comparative transcriptomic analyses on graft combinations indicated that a subclade of β-1,4-glucanases secreted into the extracellular region facilitates cell wall reconstruction near the graft interface. Grafting was promoted by overexpression of the β-1,4-glucanase. Using Nicotiana stem as an interscion, we produced tomato fruits on rootstocks from other plant families. These findings demonstrate that the process of cell-cell adhesion is a potential target to enhance plant grafting techniques.
      (Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.)
    • Comments:
      Comment in: Science. 2020 Aug 7;369(6504):618-619. (PMID: 32764051)
    • Accession Number:
      0 (Plant Proteins)
      EC 3.2.1.4 (Cellulase)
    • Publication Date:
      Date Created: 20200809 Date Completed: 20201021 Latest Revision: 20231213
    • Publication Date:
      20240829
    • Accession Number:
      10.1126/science.abc3710
    • Accession Number:
      32764072