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Screening active components from Rubus amabilis for pancreatic β-cells protection.
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- Additional Information
- Source:
Publisher: Taylor & Francis Country of Publication: England NLM ID: 9812552 Publication Model: Print Cited Medium: Internet ISSN: 1744-5116 (Electronic) Linking ISSN: 13880209 NLM ISO Abbreviation: Pharm Biol Subsets: MEDLINE
- Publication Information:
Publication: [London] : Taylor & Francis
Original Publication: Lisse, the Netherlands : Swets & Zeitlinger, c1998-
- Subject Terms:
- Abstract:
Context: Rubus species (Rosaceae) have been used in folk medicine to treat diabetes due to their hypoglycaemic activity.
Objective: To screen the active components that act as hypoglycaemic agents in Rubus amabilis Focke and the underlying mechanisms.
Materials and Methods: Aqueous stem extract of R. amabilis was incubated with MIN6 β-cells, PBS was used as the blank control. Then the cells were washed, cell membrane-bound components were dissociated and identified by UPLC/MS. Total procyanidins (PCs) in R. amabilis was enriched and the cytotoxicity and anti-proliferation on β-cell were evaluated by MTT assay. PCs at 25, 50, and 75 μg/mL was applied for 24 h to determine its effects on palmitate (PA)-induced apoptosis and GSIS. Western blotting was employed to detect the protein expression of PI3K/Akt/FoxO1 signalling. The antioxidant indices were also measured.
Results: β-Cell membrane-bound components were identified as three procyanidin B dimers and a C trimer. PCs showed no significant cytotoxicity up to a concentrations of 100 μg/mL. PCs treatment reversed the elevated apoptosis rate and impaired GSIS induced by PA. PCs markedly decreased the intracellular ROS and MDA production and increased the SOD activity. Moreover, PCs promoted the phosphorylation of Akt and FoxO1, and regulated Pdx-1 and Bax expression in MIN6 cells. Discussion and conclusion: The active components that act as hypoglycaemic agents in R. amabilis are procyanidins, which protected MIN6 cells against PA-induced apoptosis by activating PI3K/Akt/FoxO1 signalling. These results indicate that β-cell extraction, combined with UPLC/MS, is a valid method for screening antidiabetic components from herbal medicines.
- References:
J Pharm Biomed Anal. 2007 Jun 28;44(2):439-43. (PMID: 17276646)
J Pharm Biomed Anal. 2014 Dec;101:141-50. (PMID: 24972757)
Mol Carcinog. 2016 Dec;55(12):2196-2209. (PMID: 26774105)
Molecules. 2012 Feb 06;17(2):1468-82. (PMID: 22310168)
Obstet Gynecol. 2016 Dec;128(6):1421-1424. (PMID: 27824754)
Mediators Inflamm. 2013;2013:750540. (PMID: 23737653)
Apoptosis. 2015 Nov;20(11):1420-32. (PMID: 26330141)
Phytother Res. 1999 Mar;13(2):91-4. (PMID: 10190178)
Molecules. 2017 Jul 05;22(7):. (PMID: 28678204)
Food Chem. 2013 May 1;138(1):524-30. (PMID: 23265520)
Arch Pharm Res. 2015 Jun;38(6):1044-53. (PMID: 25156360)
Curr Med Chem. 2015;22(1):39-50. (PMID: 25245512)
Am J Med. 2000 Apr 17;108 Suppl 6a:2S-8S. (PMID: 10764844)
Drug Discov Ther. 2015 Dec;9(6):391-6. (PMID: 26781923)
J Sci Food Agric. 2017 Aug;97(11):3576-3583. (PMID: 28098355)
Biosci Biotechnol Biochem. 2013;77(4):888-91. (PMID: 23563558)
PLoS One. 2014 Sep 08;9(9):e107279. (PMID: 25198676)
Endocrinology. 2004 Nov;145(11):4985-90. (PMID: 15271880)
J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Jan 1;912:85-92. (PMID: 23262196)
Food Funct. 2017 Mar 22;8(3):1052-1060. (PMID: 28134947)
J Agric Food Chem. 2016 Nov 23;64(46):8857-8865. (PMID: 27792335)
Biochim Biophys Acta. 2011 Nov;1813(11):1978-86. (PMID: 21440011)
Bioorg Med Chem Lett. 2006 Mar 1;16(5):1380-3. (PMID: 16359864)
J Sci Food Agric. 2010 Jul;90(9):1473-8. (PMID: 20549799)
J Pharm Biomed Anal. 2005 Jul 15;38(4):664-9. (PMID: 15967294)
J Food Sci. 2018 Oct;83(10):2622-2628. (PMID: 30221772)
Chin J Nat Med. 2016 May;14(5):377-81. (PMID: 27478101)
Acta Pharmacol Sin. 2016 Aug;37(8):1083-90. (PMID: 27238208)
Trends Cell Biol. 2011 Jul;21(7):424-31. (PMID: 21481590)
Zhong Yao Cai. 2016 Sep;39(9):2019-23. (PMID: 30209904)
Br J Pharmacol. 2017 Jun;174(11):1209-1225. (PMID: 28071785)
Biochim Biophys Acta. 2016 Dec;1863(12):2977-2992. (PMID: 27646922)
Curr Med Chem. 2004 Jun;11(11):1501-12. (PMID: 15180580)
J Agric Food Chem. 2014 Jun 4;62(22):5038-45. (PMID: 24815044)
Cell Biochem Funct. 2012 Mar;30(2):151-7. (PMID: 22095860)
Int J Mol Sci. 2014 Nov 06;15(11):20382-402. (PMID: 25383680)
J Nutr Biochem. 2012 Dec;23(12):1565-72. (PMID: 22444499)
Diabetes. 2017 Nov;66(11):2738-2740. (PMID: 29061659)
J Cell Biochem. 2014 Jun;115(6):1166-75. (PMID: 24415347)
Diabetes. 2005 Dec;54 Suppl 2:S97-107. (PMID: 16306347)
Diabetes. 2003 Jan;52(1):102-10. (PMID: 12502499)
- Contributed Indexing:
Keywords: PI3K/Akt/FoxO1 signalling pathway; Procyanidin; apoptosis; cell membrane chromatography
- Accession Number:
0 (Forkhead Box Protein O1)
0 (Foxo1 protein, mouse)
0 (Hypoglycemic Agents)
0 (Plant Extracts)
0 (Proanthocyanidins)
EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
- Publication Date:
Date Created: 20200714 Date Completed: 20210521 Latest Revision: 20210521
- Publication Date:
20240829
- Accession Number:
PMC7470145
- Accession Number:
10.1080/13880209.2020.1787467
- Accession Number:
32659127
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