Hyperhomocysteinemia and Low Folate and Vitamin B12 Are Associated with Vascular Dysfunction and Impaired Nitric Oxide Sensitivity in Morbidly Obese Patients.

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  • Additional Information
    • Source:
      Publisher: MDPI Publishing Country of Publication: Switzerland NLM ID: 101521595 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6643 (Electronic) Linking ISSN: 20726643 NLM ISO Abbreviation: Nutrients Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI Publishing
    • Subject Terms:
      Folic Acid/*blood ; Hyperhomocysteinemia/*epidemiology ; Nitric Oxide/*metabolism ; Obesity, Morbid/*epidemiology ; Vascular Diseases/*epidemiology ; Vitamin B 12/*blood; Adult ; Alcohol Drinking/epidemiology ; Arterioles ; Bariatric Surgery/methods ; Brachial Artery ; Female ; Folic Acid Deficiency/epidemiology ; Homocysteine/blood ; Humans ; Male ; Nutritional Status ; Obesity, Morbid/surgery ; Vitamin B 12 Deficiency/epidemiology
    • Abstract:
      There is a high prevalence of hyperhomocysteinemia that has been linked to high cardiovascular risk in obese individuals and could be attributed to poor nutritional status of folate and vitamin B12. We sought to examine the association between blood homocysteine (Hcy) folate, and vitamin B12 levels and vascular dysfunction in morbidly obese adults using novel ex vivo flow-induced dilation (FID) measurements of isolated adipose tissue arterioles. Brachial artery flow-mediated dilation (FMD) was also measured. Subcutaneous and visceral adipose tissue biopsies were obtained from morbidly obese individuals and non-obese controls. Resistance arterioles were isolated in which FID, acetylcholine-induced dilation (AChID), and nitric oxide (NO) production were measured in the absence or presence of the NO synthase inhibitor, L-NAME, Hcy, or the superoxide dismutase mimetic, TEMPOL. Our results demonstrated that plasma Hcy concentrations were significantly higher, while folate, vitamin B12, and NO were significantly lower in obese subjects compared to controls. Hcy concentrations correlated positively with BMI, fat %, and insulin levels but not with folate or vitamin B12. Brachial and arteriolar vasodilation were lower in obese subjects, positively correlated with folate and vitamin B12, and inversely correlated with Hcy. Arteriolar NO measurements and sensitivity to L-NAME were lower in obese subjects compared to controls. Finally, Hcy incubation reduced arteriolar FID and NO sensitivity, an effect that was abolished by TEMPOL. In conclusion, these data suggest that high concentrations of plasma Hcy and low concentrations of folate and vitamin B12 could be independent predictors of vascular dysfunction in morbidly obese individuals.
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    • Grant Information:
      K99 HL140049 United States HL NHLBI NIH HHS; R00 HL140049 United States HL NHLBI NIH HHS; HL095701 United States NH NIH HHS; HL130513 United States NH NIH HHS; K99HL140049 United States NH NIH HHS; 4 R00 HL140049-03 United States NH NIH HHS
    • Contributed Indexing:
      Keywords: bariatric surgery; folate; homocysteine; nitric oxide; obesity; vascular dysfunction; vitamin B12
    • Accession Number:
      0LVT1QZ0BA (Homocysteine)
      31C4KY9ESH (Nitric Oxide)
      935E97BOY8 (Folic Acid)
      P6YC3EG204 (Vitamin B 12)
    • Publication Date:
      Date Created: 20200711 Date Completed: 20210301 Latest Revision: 20210301
    • Publication Date:
      20240829
    • Accession Number:
      PMC7400872
    • Accession Number:
      10.3390/nu12072014
    • Accession Number:
      32645905