A long noncoding RNA CHAIR protects the heart from pathological stress.

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  • Additional Information
    • Source:
      Publisher: Portland Press on behalf of the Medical Research Society and the Biochemical Society Country of Publication: England NLM ID: 7905731 Publication Model: Print Cited Medium: Internet ISSN: 1470-8736 (Electronic) Linking ISSN: 01435221 NLM ISO Abbreviation: Clin Sci (Lond) Subsets: MEDLINE
    • Publication Information:
      Publication: London : Portland Press on behalf of the Medical Research Society and the Biochemical Society
      Original Publication: London, Medical Research Society.
    • Subject Terms:
      Cardiomegaly/*metabolism ; Heart Failure/*metabolism ; Myocytes, Cardiac/*metabolism ; RNA, Long Noncoding/*metabolism; Animals ; Cardiomegaly/genetics ; Cardiomegaly/pathology ; DNA (Cytosine-5-)-Methyltransferases/genetics ; DNA (Cytosine-5-)-Methyltransferases/metabolism ; DNA Methyltransferase 3A ; Female ; Heart/growth & development ; Heart Failure/genetics ; Heart Failure/pathology ; Humans ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; RNA, Long Noncoding/genetics
    • Abstract:
      Mammalian genomes have been found to be extensively transcribed. In addition to classic protein coding genes, a large numbers of long noncoding genes (lncRNAs) have been identified, while their functions, especially in heart diseases, remain to be established. We hypothesized that heart failure progression is controlled by tissue-specific lncRNAs. In the present study, we found that the cardiac-enriched lncRNA 4632428C04Rik, named as cardiomyocyte hypertrophic associated inhibitory RNA (CHAIR), is dynamically regulated during heart development, is expressed at low levels in embryonic hearts and accumulated at high levels in adult hearts. More interestingly, the lncRNA was down-regulated during cardiac hypertrophy and failure both in mice and humans. Importantly, loss of lncRNA CHAIR has no effects on normal hearts, whereas it results in accelerated heart function decline, increased hypertrophy, and exacerbated heart failure in response to stress. In contrast, restoring the expression of lncRNA CHAIR rescued the hearts from hypertrophy and failure. DNMT3A was recruited to CHAIR promoter during heart failure to suppress its expression. Reciprocally, CHAIR interacted with DNMT3A to inhibit its DNA-binding activity. Taken together, our data revealed a new cardioprotective lncRNA that represses heart failure through an epigenetic mechanism.
      (© 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.)
    • Contributed Indexing:
      Keywords: CHAIR; Cardiac hypertrophy; DNMT3A; Long noncoding RNA
    • Accession Number:
      0 (DNMT3A protein, human)
      0 (Dnmt3a protein, mouse)
      0 (RNA, Long Noncoding)
      EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases)
      EC 2.1.1.37 (DNA Methyltransferase 3A)
    • Publication Date:
      Date Created: 20200710 Date Completed: 20210201 Latest Revision: 20211204
    • Publication Date:
      20231215
    • Accession Number:
      10.1042/CS20200149
    • Accession Number:
      32643756