Polyphenols from Toona sinensiss Seeds Alleviate Neuroinflammation Induced by 6-Hydroxydopamine Through Suppressing p38 MAPK Signaling Pathway in a Rat Model of Parkinson's Disease.

Publisher: Kluwer Academic/Plenum Publishers Country of Publication: United States NLM ID: 7613461 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-6903 (Electronic) Linking ISSN: 03643190 NLM ISO Abbreviation: Neurochem Res Subsets: MEDLINE

Publication: 1999- : New York, NY : Kluwer Academic/Plenum Publishers
Original Publication: New York, Plenum Press

Anti-Inflammatory Agents/*therapeutic use ; Inflammation/*drug therapy ; MAP Kinase Signaling System/*drug effects ; Oxidopamine/*toxicity ; Parkinson Disease, Secondary/*drug therapy ; Polyphenols/*therapeutic use; Animals ; Astrocytes/drug effects ; Cyclooxygenase 2/metabolism ; Dopaminergic Neurons/drug effects ; Inflammation/chemically induced ; Male ; Microglia/drug effects ; Nitric Oxide Synthase Type II/metabolism ; PC12 Cells ; Parkinson Disease, Secondary/chemically induced ; Rats ; Rats, Sprague-Dawley ; Seeds/chemistry ; Substantia Nigra/drug effects ; Substantia Nigra/metabolism ; Toona/chemistry ; Tumor Necrosis Factor-alpha/metabolism

Polyphenols from Toona sinensis seeds (PTSS) have demonstrated anti-inflammatory effects in various diseases, while the anti-neuroinflammatory effects still remain to be investigated. We aimed to investigate the effects of PTSS on Parkinson's disease and underlying mechanisms using a rat model. We employed 6-hydroxydopamine (6-OHDA) to male Sprague Dawley (SD) rats and PC12 cells to construct the in vivo and vitro models of PD and dopaminergic (DA) neuron injury, respectively. Cell viability was detected by cell counting kit-8 (CCK-8) assay and protein levels of inflammatory mediators and some p38 MAPK pathway molecules were investigated by immunohistochemistry and Western blot analyses. The results showed that 6-OHDA significantly increased protein levels of inflammatory mediators, such as cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and tumor necrosis factor α (TNF-α), which could be reversed by PTSS through suppressing the p38 MAPK pathway. The anti-inflammatory effects of PTSS were significantly enhanced by the specific p38 inhibitor of SB203580 in vitro. The present work suggests that PTSS can exert anti-inflammatory effects on PD models, which may be attributed to the suppression of p38 MAPK signaling pathway.

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81870943 National Natural Science Foundation of China; ZR2014HL043 Natural Science Foundation of Shandong Province

Keywords: Neuroinflammation; Parkinson’s disease; Polyphenols from toona sinensis seeds; p38 MAPK

0 (Anti-Inflammatory Agents)
0 (Polyphenols)
0 (Tumor Necrosis Factor-alpha)
8HW4YBZ748 (Oxidopamine)
EC 1.14.13.39 (Nitric Oxide Synthase Type II)
EC 1.14.13.39 (Nos2 protein, rat)
EC 1.14.99.1 (Cyclooxygenase 2)
EC 1.14.99.1 (Ptgs2 protein, rat)

Date Created: 20200620 Date Completed: 20210608 Latest Revision: 20210617

20221213

10.1007/s11064-020-03067-2

32556929