New multilocus sequence typing primers to enable genotyping of AD hybrids within the Cryptococcus neoformans species complex.

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  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 9815835 Publication Model: Print Cited Medium: Internet ISSN: 1460-2709 (Electronic) Linking ISSN: 13693786 NLM ISO Abbreviation: Med Mycol Subsets: MEDLINE
    • Publication Information:
      Publication: 2014- : Oxford : Oxford University Press
      Original Publication: Oxford : Blackwell Science, c1998-
    • Subject Terms:
      Genotyping Techniques* ; Mycological Typing Techniques*; Chimera/*genetics ; Cryptococcus neoformans/*classification ; Cryptococcus neoformans/*genetics ; Multilocus Sequence Typing/*methods; Europe ; Genetic Variation ; Genotype ; Humans ; Phylogeny
    • Abstract:
      Although AD hybrids within the Cryptococcus neoformans species complex represent about 20% of the isolates identified in Europe, phylogenetic and population genetic studies are lacking due to the inability to use the standardized typing method. The aim of the present study was to design new molecular type specific primers in order to apply the standard ISHAM consensus multilocus sequence typing (MLST) scheme to AD hybrids. The new primers are able to specifically amplify VNI and VNIV alleles of the seven MLST loci in both haploid and diploid or aneuploid hybrid strains. This study forms the basis for future molecular epidemiology studies of AD hybrids.
      Lay Abstract: We designed and tested new specific primers to amplify the two alleles of each of the seven MLST loci in C. neoformans species complex hybrids. The sequences obtained from hybrids can be compared with those present in the Cryptococcus global MLST database for future molecular epidemiology studies.
      (© The Author(s) 2020. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.)
    • Contributed Indexing:
      Keywords: Cryptococcus neoformans; AD hybrids; MLST; MLST database; sequence type
    • Publication Date:
      Date Created: 20200609 Date Completed: 20210526 Latest Revision: 20220417
    • Publication Date:
      20221213
    • Accession Number:
      10.1093/mmy/myaa047
    • Accession Number:
      32511709