Molecular cloning of crustacean hyperglycemic hormone (CHH) family members (CHH, molt-inhibiting hormone and mandibular organ-inhibiting hormone) and their expression levels in the Jonah crab, Cancer borealis.

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  • Author(s): Sook Chung J;Sook Chung J; Christie A; Christie A; Flynn E; Flynn E
  • Source:
    General and comparative endocrinology [Gen Comp Endocrinol] 2020 Sep 01; Vol. 295, pp. 113522. Date of Electronic Publication: 2020 May 31.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Academic Press Country of Publication: United States NLM ID: 0370735 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-6840 (Electronic) Linking ISSN: 00166480 NLM ISO Abbreviation: Gen Comp Endocrinol Subsets: MEDLINE
    • Publication Information:
      Publication: New York, NY : Academic Press
      Original Publication: New York.
    • Subject Terms:
      Gene Expression Regulation*; Arthropod Proteins/*genetics ; Brachyura/*genetics ; Invertebrate Hormones/*genetics ; Nerve Tissue Proteins/*genetics; Amino Acid Sequence ; Animal Structures/metabolism ; Animals ; Chromatography, High Pressure Liquid ; Chromatography, Reverse-Phase ; Cloning, Molecular ; DNA, Complementary/metabolism ; Female ; Hemolymph/metabolism ; Male ; Neuropeptides/chemistry ; Neuropeptides/genetics ; Neuropeptides/metabolism ; Neurosecretory Systems/metabolism
    • Abstract:
      The crustacean hyperglycemic hormone (CHH) neuropeptide family has multiple functions in the regulation of hemolymph glucose levels, molting, ion, and water balance and reproduction. In crab species, three neuroendocrine tissues: the eyestalk ganglia (medulla terminalis X-organ and -sinus gland = ES), the pericardial organ (PO), and guts synthesize a tissue-specific isoforms of CHH neuropeptides. Recently the presence of the mandibular organ-inhibiting hormone (MOIH) was reported in the stomatogastric nervous system (STNS) that regulates the rhythmic muscle movements in esophagus, cardiac sac, gastric and pyloric ports of the foregut. In this study, we aimed to determine the presence of a tissue-specific CHH isoform in the Jonah crab, Cancer borealis using PCR with degenerate primers and 5', 3' rapid amplification of cDNA ends (RACE) in the ES. PO, and STNS. The analysis of CHH sequences shows that C. borealis has one type of CHH isoform, unlike other crab species. We also isolated the cDNA sequence of molt-inhibiting hormone (MIH) in the ES and MOIH in the ES and STNS. The presence of CHH, MOIH and MIH in the sinus gland of adult females and males is confirmed by using a dot-blot assay with the putative peaks collected from RP-HPLC and anti-Cancer sera for CHH, MIH, and MOIH. The present of crustacean female sex hormone (CFSH) in the sinus gland of adult females was examined with a dot-blot assay with anti-Callinectes CFSH serum. Levels of CHH, MOIH, and MIH in the sinus gland and their expressions in the eyestalk ganglia are estimated in the adult males, where CHH is the predominant form among these neuropeptides.
      (Copyright © 2020 Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: CHH; CHH neuropeptide family; Cancer crabs; Expression; MIH; MOIH
    • Accession Number:
      0 (Arthropod Proteins)
      0 (DNA, Complementary)
      0 (Invertebrate Hormones)
      0 (Nerve Tissue Proteins)
      0 (Neuropeptides)
      0 (hyperglycemic hormone, crustacean)
      0 (molt-inhibiting hormone)
    • Publication Date:
      Date Created: 20200604 Date Completed: 20201218 Latest Revision: 20201218
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/j.ygcen.2020.113522
    • Accession Number:
      32492383